EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational cycle of a protease-containing ABC transporter in lipid nanodiscs reveals the mechanism of cargo-protein coupling.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 9055, Year 2024
掲載日	2024年10月20日
著者	Ruojing Zhang / Kevin L Jagessar / Matthew Brownd / Adithya Polasa / Richard A Stein / Mahmoud Moradi / Erkan Karakas / Hassane S Mchaourab /
PubMed 要旨	Protease-containing ABC transporters (PCATs) couple the energy of ATP hydrolysis to the processing and export of diverse cargo proteins across cell membranes to mediate antimicrobial resistance and ...Protease-containing ABC transporters (PCATs) couple the energy of ATP hydrolysis to the processing and export of diverse cargo proteins across cell membranes to mediate antimicrobial resistance and quorum sensing. Here, we combine biochemical analysis, single particle cryoEM, and DEER spectroscopy in lipid bilayers along with computational analysis to illuminate the structural and energetic underpinnings of coupled cargo protein export. Our integrated investigation uncovers competitive interplay between nucleotides and cargo protein binding that ensures the latter's orderly processing and subsequent transport. The energetics of cryoEM structures in lipid bilayers are congruent with the inferred mechanism from ATP turnover analysis and reveal a snapshot of a high-energy outward-facing conformation that provides an exit pathway into the lipid bilayer and/or the extracellular side. DEER investigation of the core ABC transporter suggests evolutionary tuning of the energetic landscape to fulfill the function of substrate processing prior to export.
リンク	Nat Commun / PubMed:39428489 / PubMed Central
手法	EM (単粒子)
解像度	2.96 - 3.48 Å
構造データ	43393 8vow EMDB-43393, PDB-8vow: Cryo-EM structure of the ABC transporter PCAT1 cysteine-free core bound with MgATP and Vi 手法: EM (単粒子) / 解像度: 3.44 Å 43394 8vox EMDB-43394, PDB-8vox: Cryo-EM structure of the ABC transporter PCAT1 cysteine-free core bound with MgADP 手法: EM (単粒子) / 解像度: 3.07 Å 43396 8voz EMDB-43396, PDB-8voz: Cryo-EM structure of the ABC transporter PCAT1 cysteine-free core bound with MgADP and substrate 手法: EM (単粒子) / 解像度: 3.03 Å 43398 8vp1 EMDB-43398, PDB-8vp1: Cryo-EM structure of the ABC transporter PCAT1 cysteine-free core bound with ATP 手法: EM (単粒子) / 解像度: 3.48 Å 43400 8vp3 EMDB-43400, PDB-8vp3: Cryo-EM structure of the ABC transporter PCAT1 bound with MgADP and Substrate 手法: EM (単粒子) / 解像度: 2.96 Å 43401 8vp5 EMDB-43401, PDB-8vp5: Cryo-EM structure of the ABC transporter PCAT1 bound with ADP and Substrate 手法: EM (単粒子) / 解像度: 3.18 Å 43402 8vp6 EMDB-43402, PDB-8vp6: Cryo-EM structure of the ABC transporter PCAT1 bound with Mg_class_2 手法: EM (単粒子) / 解像度: 2.97 Å 43403 8vp8 EMDB-43403, PDB-8vp8: Cryo-EM structure of the ABC transporter PCAT1 bound with ATP and Substrate 手法: EM (単粒子) / 解像度: 3.37 Å 43404 8vp9 EMDB-43404, PDB-8vp9: Cryo-EM structure of the cysteine-free ABC transporter PCAT1 bound with ADP and Substrate 手法: EM (単粒子) / 解像度: 3.18 Å 43405 8vpa EMDB-43405, PDB-8vpa: Cryo-EM structure of the ABC transporter PCAT1 bound with ADP 手法: EM (単粒子) / 解像度: 3.11 Å 43406 8vpb EMDB-43406, PDB-8vpb: Cryo-EM structure of the ABC transporter PCAT1 bound with MgADP_Class_2 手法: EM (単粒子) / 解像度: 3.23 Å 44015 9azl EMDB-44015, PDB-9azl: Cryo-EM structure of the ABC transporter PCAT1 bound with MgADP class1 手法: EM (単粒子) / 解像度: 2.99 Å 44402 9baa EMDB-44402, PDB-9baa: Cryo-EM structure of the ABC transporter PCAT1 bound with Mg_class_1 手法: EM (単粒子) / 解像度: 3.39 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-VO4: VANADATE ION PDB-1acx: ACTINOXANTHIN STRUCTURE AT THE ATOMIC LEVEL (RUSSIAN) ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質*YM
由来	acetivibrio thermocellus atcc 27405 (バクテリア)
キーワード	TRANSPORT PROTEIN / ABC transporter / Nucleotide / Membrane Protein