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TitleStructures of Mature and Urea-Treated Empty Bacteriophage T5: Insights into Siphophage Infection and DNA Ejection.
Journal, issue, pagesInt J Mol Sci, Vol. 25, Issue 15, Year 2024
Publish dateAug 3, 2024
AuthorsYuning Peng / Huanrong Tang / Hao Xiao / Wenyuan Chen / Jingdong Song / Jing Zheng / Hongrong Liu /
PubMed AbstractT5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, ...T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine the structures of mature T5 (a laboratory-adapted, fiberless T5 mutant) and urea-treated empty T5 (lacking the tip complex) at near-atomic resolutions. Atomic models of the head, connector complex, tail tube, and tail tip were built for mature T5, and atomic models of the connector complex, comprising the portal protein pb7, adaptor protein p144, and tail terminator protein p142, were built for urea-treated empty T5. Our findings revealed that the aforementioned proteins did not undergo global conformational changes before and after DNA ejection, indicating that these structural features were conserved among most myophages and siphophages. The present study elucidates the underlying mechanisms of siphophage infection and DNA ejection.
External linksInt J Mol Sci / PubMed:39126049 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.4 - 4.8 Å
Structure data

60511

8zvi

EMDB-60511, PDB-8zvi:
Structure of the bacteriophage T5 capsid
Method: EM (single particle) / Resolution: 3.4 Å

60672

9ilp

EMDB-60672, PDB-9ilp:
Structure of the bacteriophage T5 portal complex
Method: EM (single particle) / Resolution: 3.4 Å

60675

9ilv

EMDB-60675, PDB-9ilv:
Structure of the bacteriophage T5 connector complex
Method: EM (single particle) / Resolution: 4.8 Å

60689

9imh

EMDB-60689, PDB-9imh:
Structure of urea-treated empty bacteriophage T5 connector complex
Method: EM (single particle) / Resolution: 4.2 Å

60695

9imv

EMDB-60695, PDB-9imv:
Structure of the urea-treated empty bacteriophage T5 portal complex
Method: EM (single particle) / Resolution: 4.0 Å

60712

9iny

EMDB-60712, PDB-9iny:
Structure of bacteriophage T5 tail tube
Method: EM (helical sym.) / Resolution: 3.6 Å

60750

9ioz

EMDB-60750, PDB-9ioz:
Structure of the bacteriophage T5 tail tip complex
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • escherichia phage t5 (virus)
KeywordsSTRUCTURAL PROTEIN / Complex / VIRAL PROTEIN

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