+検索条件
-Structure paper
タイトル | Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 4535, Year 2024 |
掲載日 | 2024年5月28日 |
著者 | Zhiyuan Mao / Xingyue Li / Zhenhua Li / Liangliang Shen / Xiaoyi Li / Yanyan Yang / Wenda Wang / Tingyun Kuang / Jian-Ren Shen / Guangye Han / |
PubMed 要旨 | Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin ...Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery. |
リンク | Nat Commun / PubMed:38806516 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.47 - 2.84 Å |
構造データ | EMDB-37414, PDB-8wb4: EMDB-38419, PDB-8xkl: |
化合物 | ChemComp-8CT: ChemComp-CLA: ChemComp-KC2: ChemComp-II0: ChemComp-II3: ChemComp-IHT: ChemComp-LMG: ChemComp-LHG: ChemComp-SQD: ChemComp-OEX: ChemComp-PHO: ChemComp-CL: ChemComp-PL9: ChemComp-BCT: ChemComp-DGD: ChemComp-FE2: ChemComp-HEM: |
由来 |
|
キーワード | PHOTOSYNTHESIS / Photosystem II / STRUCTURAL PROTEIN |