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- PDB-8wb4: Structure of PSII-ACPII supercomplex from cryptophyte algae -

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Entry
Database: PDB / ID: 8wb4
TitleStructure of PSII-ACPII supercomplex from cryptophyte algae
Components
  • (Cytochrome b559 subunit ...) x 2
  • (Photosystem II ...) x 21
  • ACPII-1
  • ACPII-2
  • ACPII-3
  • ACPII-4
  • ACPII-5
  • ACPII-6
  • CCPII-S
KeywordsPHOTOSYNTHESIS / Photosystem II
Function / homology
Function and homology information


thylakoid membrane / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / chlorophyll binding ...thylakoid membrane / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / photosynthetic electron transport in photosystem II / photosynthesis / respiratory electron transport chain / oxidoreductase activity / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding
Similarity search - Function
Photosystem II PsbV, cytochrome c-550 precursor / Photosystem II cytochrome c-550 precursor / Cytochrome c-550 domain / Cytochrome c-550 domain / Photosystem II PsbJ / Photosystem II PsbJ superfamily / PsbJ / Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbX, type 1 subfamily ...Photosystem II PsbV, cytochrome c-550 precursor / Photosystem II cytochrome c-550 precursor / Cytochrome c-550 domain / Cytochrome c-550 domain / Photosystem II PsbJ / Photosystem II PsbJ superfamily / PsbJ / Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbX, type 1 subfamily / Photosystem II reaction centre M protein (PsbM) / Photosystem II PsbM superfamily / Photosystem II PsbM / Photosystem II PsbZ, reaction centre / Photosystem II PsbZ superfamily / YCF9 / Photosystem II PsbX / Photosystem II reaction centre X protein (PsbX) / Photosystem II CP43 reaction centre protein superfamily / Photosystem II PsbT / Photosystem II PsbL / Photosystem II PsbL superfamily / Photosystem II PsbT superfamily / Photosystem II reaction centre T protein / PsbL protein / Photosystem II CP43 reaction centre protein / Photosystem II PsbK / Photosystem II PsbK superfamily / Photosystem II 4 kDa reaction centre component / Photosystem II CP47 reaction centre protein / Photosystem II PsbI / Photosystem II PsbI superfamily / Photosystem II reaction centre I protein (PSII 4.8 kDa protein) / Photosystem II reaction centre protein H / Photosystem II protein D1 / Photosystem II D2 protein / Photosystem II cytochrome b559, conserved site / Photosystem II cytochrome b559, alpha subunit / Photosystem II cytochrome b559, beta subunit / Photosystem II cytochrome b559, N-terminal / Photosystem II cytochrome b559, alpha subunit, lumenal region / Photosystem II reaction centre protein H superfamily / Photosystem II cytochrome b559, alpha subunit superfamily / Cytochrome b559, alpha (gene psbE) and beta (gene psbF)subunits / Lumenal portion of Cytochrome b559, alpha (gene psbE) subunit / Photosystem II 10 kDa phosphoprotein / Cytochrome b559 subunits heme-binding site signature. / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature. / Cytochrome c family profile. / Cytochrome c-like domain / Cytochrome c-like domain superfamily
Similarity search - Domain/homology
Chem-8CT / BICARBONATE ION / CHLOROPHYLL A / DIGALACTOSYL DIACYL GLYCEROL (DGDG) / : / PROTOPORPHYRIN IX CONTAINING FE / Chem-IHT / Chem-II0 / Chem-II3 / Chlorophyll c2 ...Chem-8CT / BICARBONATE ION / CHLOROPHYLL A / DIGALACTOSYL DIACYL GLYCEROL (DGDG) / : / PROTOPORPHYRIN IX CONTAINING FE / Chem-IHT / Chem-II0 / Chem-II3 / Chlorophyll c2 / 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / CA-MN4-O5 CLUSTER / PHEOPHYTIN A / Chem-PL9 / Chem-SQD / Photosystem II cytochrome c550 / Photosystem II CP43 reaction center protein / Photosystem II D2 protein / Photosystem II protein D1 / Cytochrome b559 subunit beta / Photosystem II reaction center protein J / Photosystem II reaction center protein L / Photosystem II reaction center protein X / Photosystem II reaction center protein Psb30 / Cytochrome b559 subunit alpha / Photosystem II reaction center protein I / Photosystem II reaction center protein Z / Photosystem II reaction center protein K / Photosystem II reaction center protein T / Photosystem II CP47 reaction center protein / Photosystem II reaction center protein H / Photosystem II reaction center M
Similarity search - Component
Biological speciesChroomonas placoidea (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.47 Å
AuthorsLi, X.Y. / Mao, Z.Y. / Shen, J.R. / Han, G.Y.
Funding support China, 1items
OrganizationGrant numberCountry
Chinese Academy of SciencesYSBR-004 China
CitationJournal: Nat Commun / Year: 2024
Title: Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea.
Authors: Zhiyuan Mao / Xingyue Li / Zhenhua Li / Liangliang Shen / Xiaoyi Li / Yanyan Yang / Wenda Wang / Tingyun Kuang / Jian-Ren Shen / Guangye Han /
Abstract: Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin ...Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery.
History
DepositionSep 8, 2023Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Jun 5, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
m: Photosystem II reaction center M
M: Photosystem II reaction center M
1: ACPII-1
2: ACPII-2
3: ACPII-3
4: ACPII-4
5: ACPII-5
6: ACPII-6
A: Photosystem II protein D1
D: Photosystem II D2 protein
E: Cytochrome b559 subunit alpha
F: Cytochrome b559 subunit beta
H: Photosystem II reaction center protein H
I: Photosystem II reaction center protein I
J: Photosystem II reaction center protein J
K: Photosystem II reaction center protein K
O: Photosystem II reaction center protein O
Q: Photosystem II reaction center protein Q
R: Photosystem II reaction center protein R
S: CCPII-S
T: Photosystem II reaction center protein T
U: Photosystem II reaction center protein U
V: Photosystem II cytochrome c550
X: Photosystem II reaction center X protein
Y: Photosystem II reaction center protein Ycf12
Z: Photosystem II reaction center protein Z
b: Photosystem II CP47 reaction center protein
c: Photosystem II CP43 reaction center protein
d: Photosystem II D2 protein
e: Cytochrome b559 subunit alpha
f: Cytochrome b559 subunit beta
g: Photosystem II reaction center protein G
i: Photosystem II reaction center protein I
j: Photosystem II reaction center protein J
k: Photosystem II reaction center protein K
l: Photosystem II reaction center protein L
o: Photosystem II reaction center protein O
r: Photosystem II reaction center protein R
u: Photosystem II reaction center protein U
v: Photosystem II cytochrome c550
x: Photosystem II reaction center X protein
y: Photosystem II reaction center protein Ycf12
z: Photosystem II reaction center protein Z
0: ACPII-4
7: ACPII-1
8: ACPII-2
9: ACPII-3
C: Photosystem II CP43 reaction center protein
G: Photosystem II reaction center protein G
L: Photosystem II reaction center protein L
P: ACPII-6
a: Photosystem II protein D1
h: Photosystem II reaction center protein H
p: ACPII-5
q: Photosystem II reaction center protein Q
s: CCPII-S
t: Photosystem II reaction center protein T
B: Photosystem II CP47 reaction center protein
w: Photosystem II reaction center protein W
W: Photosystem II reaction center protein W
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,332,326436
Polymers1,040,24660
Non-polymers292,079376
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Photosystem II ... , 21 types, 42 molecules mMAaDdHhIiJjKkOoQqRrTtUuVvXxYy...

#1: Protein Photosystem II reaction center M


Mass: 11880.879 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Details: Author stated: We did not find the sequence of chain m,M in the transcriptome of Chroomonas placoidea, so the sequence in Guillardia theta (strain CCMP2712) (tax id 905079) was used.
Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: L1IFP3
#8: Protein Photosystem II protein D1 / PSII D1 protein / Photosystem II Q(B) protein


Mass: 39590.910 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI41, photosystem II
#9: Protein Photosystem II D2 protein / PSII D2 protein / Photosystem Q(A) protein


Mass: 39216.812 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI37, photosystem II
#12: Protein Photosystem II reaction center protein H / PSII-H


Mass: 7519.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AIE4
#13: Protein/peptide Photosystem II reaction center protein I / PSII-I / PSII 4.4 kDa protein


Mass: 4424.081 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI80
#14: Protein/peptide Photosystem II reaction center protein J / PSII-J


Mass: 3964.609 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI54
#15: Protein/peptide Photosystem II reaction center protein K / PSII-K


Mass: 5005.114 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AIA0
#16: Protein Photosystem II reaction center protein O


Mass: 34995.547 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#17: Protein/peptide Photosystem II reaction center protein Q


Mass: 1805.216 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#18: Protein/peptide Photosystem II reaction center protein R


Mass: 2230.741 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#20: Protein/peptide Photosystem II reaction center protein T / PSII-T


Mass: 3664.447 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AID2
#21: Protein Photosystem II reaction center protein U


Mass: 15962.294 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#22: Protein Photosystem II cytochrome c550


Mass: 17708.234 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI35
#23: Protein/peptide Photosystem II reaction center X protein


Mass: 4149.939 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI61
#24: Protein/peptide Photosystem II reaction center protein Ycf12


Mass: 3763.581 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI69
#25: Protein Photosystem II reaction center protein Z / PSII-Z


Mass: 6573.990 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI96
#26: Protein Photosystem II CP47 reaction center protein / PSII 47 kDa protein / Protein CP-47


Mass: 56354.172 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AIE3
#27: Protein Photosystem II CP43 reaction center protein / PSII 43 kDa protein / Protein CP-43


Mass: 50508.488 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI36
#28: Protein Photosystem II reaction center protein G


Mass: 5464.728 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#29: Protein/peptide Photosystem II reaction center protein L / PSII-L


Mass: 4363.087 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI58
#30: Protein Photosystem II reaction center protein W


Mass: 11994.986 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)

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Protein , 7 types, 14 molecules 172839405p6PSs

#2: Protein ACPII-1


Mass: 25151.223 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#3: Protein ACPII-2


Mass: 23471.201 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#4: Protein ACPII-3


Mass: 24055.020 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#5: Protein ACPII-4


Mass: 23734.623 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#6: Protein ACPII-5


Mass: 23762.242 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#7: Protein ACPII-6


Mass: 23923.801 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)
#19: Protein CCPII-S


Mass: 30531.848 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote)

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Cytochrome b559 subunit ... , 2 types, 4 molecules EeFf

#10: Protein Cytochrome b559 subunit alpha / PSII reaction center subunit V


Mass: 9505.693 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI74
#11: Protein/peptide Cytochrome b559 subunit beta / PSII reaction center subunit VI


Mass: 4845.769 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chroomonas placoidea (eukaryote) / References: UniProt: A0A222AI51

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Sugars , 1 types, 10 molecules

#45: Sugar
ChemComp-DGD / DIGALACTOSYL DIACYL GLYCEROL (DGDG)


Type: saccharide / Mass: 949.299 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C51H96O15 / Feature type: SUBJECT OF INVESTIGATION

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Non-polymers , 16 types, 366 molecules

#31: Chemical...
ChemComp-8CT / (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene


Mass: 536.873 Da / Num. of mol.: 24 / Source method: obtained synthetically / Formula: C40H56 / Feature type: SUBJECT OF INVESTIGATION
#32: Chemical...
ChemComp-CLA / CHLOROPHYLL A


Mass: 893.489 Da / Num. of mol.: 208 / Source method: obtained synthetically / Formula: C55H72MgN4O5 / Feature type: SUBJECT OF INVESTIGATION
#33: Chemical
ChemComp-KC2 / Chlorophyll c2


Mass: 608.926 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C35H28MgN4O5 / Feature type: SUBJECT OF INVESTIGATION
#34: Chemical...
ChemComp-II0 / (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol / Alloxanthin


Mass: 564.840 Da / Num. of mol.: 42 / Source method: obtained synthetically / Formula: C40H52O2 / Feature type: SUBJECT OF INVESTIGATION
#35: Chemical
ChemComp-II3 / (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol / Monadoxanthin


Mass: 566.856 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C40H54O2 / Feature type: SUBJECT OF INVESTIGATION
#36: Chemical
ChemComp-IHT / (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol / Allobetaxanthin


Mass: 550.856 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C40H54O / Feature type: SUBJECT OF INVESTIGATION
#37: Chemical
ChemComp-LMG / 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE


Mass: 787.158 Da / Num. of mol.: 20 / Source method: obtained synthetically / Formula: C45H86O10 / Feature type: SUBJECT OF INVESTIGATION
#38: Chemical
ChemComp-LHG / 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE


Mass: 722.970 Da / Num. of mol.: 18 / Source method: obtained synthetically / Formula: C38H75O10P / Feature type: SUBJECT OF INVESTIGATION / Comment: phospholipid*YM
#39: Chemical
ChemComp-SQD / 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL / SULFOQUINOVOSYLDIACYLGLYCEROL


Mass: 795.116 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C41H78O12S / Feature type: SUBJECT OF INVESTIGATION
#40: Chemical ChemComp-OEX / CA-MN4-O5 CLUSTER


Mass: 339.827 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CaMn4O5 / Feature type: SUBJECT OF INVESTIGATION
#41: Chemical
ChemComp-PHO / PHEOPHYTIN A


Mass: 871.200 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C55H74N4O5 / Feature type: SUBJECT OF INVESTIGATION
#42: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#43: Chemical
ChemComp-PL9 / 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / PLASTOQUINONE 9


Mass: 749.201 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C53H80O2 / Feature type: SUBJECT OF INVESTIGATION
#44: Chemical ChemComp-BCT / BICARBONATE ION


Mass: 61.017 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CHO3 / Feature type: SUBJECT OF INVESTIGATION / Comment: pH buffer*YM
#46: Chemical ChemComp-FE2 / FE (II) ION


Mass: 55.845 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe
#47: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME


Mass: 616.487 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C34H32FeN4O4 / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Structure of PSII-ACPII supercomplex from cryptophyte algae
Type: COMPLEX / Entity ID: #1-#30 / Source: NATURAL
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.47 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 305400 / Symmetry type: POINT

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