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- EMDB-38419: Structure of ACPII-CCPII from cryptophyte algae -

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Basic information

Entry
Database: EMDB / ID: EMD-38419
TitleStructure of ACPII-CCPII from cryptophyte algae
Map data
Sample
  • Complex: Structure of ACPII-CCPII from cryptophyte algae
    • Protein or peptide: x 8 types
  • Ligand: x 9 types
KeywordsPhotosystem II / PHOTOSYNTHESIS / STRUCTURAL PROTEIN
Biological speciesChroomonas placoidea (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.84 Å
AuthorsLi XY / Mao ZY / Shen JR / Han GY
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of SciencesYSBR-004 China
CitationJournal: Nat Commun / Year: 2024
Title: Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea.
Authors: Zhiyuan Mao / Xingyue Li / Zhenhua Li / Liangliang Shen / Xiaoyi Li / Yanyan Yang / Wenda Wang / Tingyun Kuang / Jian-Ren Shen / Guangye Han /
Abstract: Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin ...Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery.
History
DepositionDec 23, 2023-
Header (metadata) releaseJun 5, 2024-
Map releaseJun 5, 2024-
UpdateJun 5, 2024-
Current statusJun 5, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38419.png

Downloads & links

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Map

FileDownload / File: emd_38419.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.266
Minimum - Maximum-0.4169165 - 1.1308857
Average (Standard dev.)-0.0019910962 (±0.016036496)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 532.48 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38419_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38419_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of ACPII-CCPII from cryptophyte algae

EntireName: Structure of ACPII-CCPII from cryptophyte algae
Components
  • Complex: Structure of ACPII-CCPII from cryptophyte algae
    • Protein or peptide: ACPII-4
    • Protein or peptide: ACPII-1
    • Protein or peptide: ACPII-2
    • Protein or peptide: ACPII-3
    • Protein or peptide: Photosystem II reaction center protein G
    • Protein or peptide: ACPII-6
    • Protein or peptide: ACPII-5
    • Protein or peptide: CCPII-S
  • Ligand: CHLOROPHYLL A
  • Ligand: Chlorophyll c2
  • Ligand: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol
  • Ligand: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Supramolecule #1: Structure of ACPII-CCPII from cryptophyte algae

SupramoleculeName: Structure of ACPII-CCPII from cryptophyte algae / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8
Source (natural)Organism: Chroomonas placoidea (eukaryote)

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Macromolecule #1: ACPII-4

MacromoleculeName: ACPII-4 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 23.734623 KDa
SequenceString: AVVLAACVAS AAAFAPGASV LPRAAARATS TKGPSMQLLP LYGEGKLQGP GTQAIPGSEP PPALDGTWVG DVGFDPLGFS RVIDMRWLR EAELKHGRVC MLAATGMIVQ DIALFPGVTK TFGPAKITAL HDVAVKQGSM QQLLVWLGFL EIFGFVAIVQ M LQGSGRQP ...String:
AVVLAACVAS AAAFAPGASV LPRAAARATS TKGPSMQLLP LYGEGKLQGP GTQAIPGSEP PPALDGTWVG DVGFDPLGFS RVIDMRWLR EAELKHGRVC MLAATGMIVQ DIALFPGVTK TFGPAKITAL HDVAVKQGSM QQLLVWLGFL EIFGFVAIVQ M LQGSGRQP GDFGFDPLNC GANTDTLARR QLVELKNGRL AMIATGGMIH HFFLTGKGPI EFITTLGA

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Macromolecule #2: ACPII-1

MacromoleculeName: ACPII-1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 25.151223 KDa
SequenceString: KMLRLSILAA CLAAAQAFTS PAALLRPSTR AAVSRGPSMQ LYKDGIIQGL GVEAIPGAGR PANLDGTLVG DVGFDPLGFS NWLDLRWAR EAEIKHGRVA MLAATGMIVQ DAYKFPGFEG EFGGAAMMKL HNLAVEQGAM QQLLLWLGLL EIISGVPAII Q TLNGSERQ ...String:
KMLRLSILAA CLAAAQAFTS PAALLRPSTR AAVSRGPSMQ LYKDGIIQGL GVEAIPGAGR PANLDGTLVG DVGFDPLGFS NWLDLRWAR EAEIKHGRVA MLAATGMIVQ DAYKFPGFEG EFGGAAMMKL HNLAVEQGAM QQLLLWLGLL EIISGVPAII Q TLNGSERQ PGDFGFDPLN CGANPDTLAR RQLTELKNGR LAMIAVGGMV HHYLLVGRGP IEFITNIPNF KNPLPPF

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Macromolecule #3: ACPII-2

MacromoleculeName: ACPII-2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 23.471201 KDa
SequenceString: AMLRVALIAA ILASASAFAP MGSLGLVKSA RSGAAISPRM QSGDFSAAVP FLKRPSNLDG TLAGDVGFDP LGFSDVFDLR VLREAELKH GRFAMLAVLG FLVQEVYTFP FFPKMAPVDA HDYFVTQGGG SQIIFWISFV EIFGVVALFE LIQGKRDAGD F AFDPLGLG ...String:
AMLRVALIAA ILASASAFAP MGSLGLVKSA RSGAAISPRM QSGDFSAAVP FLKRPSNLDG TLAGDVGFDP LGFSDVFDLR VLREAELKH GRFAMLAVLG FLVQEVYTFP FFPKMAPVDA HDYFVTQGGG SQIIFWISFV EIFGVVALFE LIQGKRDAGD F AFDPLGLG KDEATLARYK VAEIKHARLA MIAIGGFIHQ FWVTKQTVLE QLGNFQSLA

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Macromolecule #4: ACPII-3

MacromoleculeName: ACPII-3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 24.05502 KDa
SequenceString: ITHSRMLRTA ILALCVAGAS AFTASPALYK SASRKAAVSG MKMQDRSYAM PFLSRPPALD GSMAGDVGFD PLGFSNYFDL KWLREAELK HGRVCMLGCL GFLVQEQANL PLPGFDNKLA TEAFFSVPAG GLWQIFFSLG AIEIITNKGK LTPGSMFTGG R APGDLDFD ...String:
ITHSRMLRTA ILALCVAGAS AFTASPALYK SASRKAAVSG MKMQDRSYAM PFLSRPPALD GSMAGDVGFD PLGFSNYFDL KWLREAELK HGRVCMLGCL GFLVQEQANL PLPGFDNKLA TEAFFSVPAG GLWQIFFSLG AIEIITNKGK LTPGSMFTGG R APGDLDFD PLNLSVDETA LRRFELAELK HARLAMIGLG GMLHQMLLTK QAPIEQLTNF KSLA

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Macromolecule #5: Photosystem II reaction center protein G

MacromoleculeName: Photosystem II reaction center protein G / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 5.464728 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #6: ACPII-6

MacromoleculeName: ACPII-6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 23.923801 KDa
SequenceString: PAMFRAALLL ACLASASAFA PGSLMMPKSA TRAAISRGPR MQANDDLAVP FLERPPMLDG SYAGDIGFDP VGFSNYFDLR WLREAELKH GRVCMLGVVG FLVQEFVTLP MFSNGVTPVD DFFVVPATGL WQIFFTIGFV EAFSNGFKLT PSDMFADDRA P GDLGFDPL ...String:
PAMFRAALLL ACLASASAFA PGSLMMPKSA TRAAISRGPR MQANDDLAVP FLERPPMLDG SYAGDIGFDP VGFSNYFDLR WLREAELKH GRVCMLGVVG FLVQEFVTLP MFSNGVTPVD DFFVVPATGL WQIFFTIGFV EAFSNGFKLT PSDMFADDRA P GDLGFDPL GCGKDPAALA RRQLVEVKNG RLAMIAFGGM LHQQLLTKQG VIEQLTNFKA IM

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Macromolecule #7: ACPII-5

MacromoleculeName: ACPII-5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 23.762242 KDa
SequenceString: ACASAAAFAP GAMPSIGKAP RAVSKTAPRM AVFPGQFSDS VPFLKQPTNL DGSYVGDVGF DPLGFSDVFD IRVLREAELK HGRIAMLAT LGMVVQEAYT FPFFDKVLPI PAHDVIVKSG GMSQILLWTS FAEIFGGIAL FQTIQGKRAP GDYSFDPLNL S ANDLEKRE ...String:
ACASAAAFAP GAMPSIGKAP RAVSKTAPRM AVFPGQFSDS VPFLKQPTNL DGSYVGDVGF DPLGFSDVFD IRVLREAELK HGRIAMLAT LGMVVQEAYT FPFFDKVLPI PAHDVIVKSG GMSQILLWTS FAEIFGGIAL FQTIQGKRAP GDYSFDPLNL S ANDLEKRE RYALAEIKHS RLAMLAFSGM VHQYFITNQG VIEQINNFRP INGFPDATFS

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Macromolecule #8: CCPII-S

MacromoleculeName: CCPII-S / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chroomonas placoidea (eukaryote)
Molecular weightTheoretical: 30.531848 KDa
SequenceString: DHKRSRMMKS LALAAVGLAV GAEAFAPTPM VGGAKLALRT SSTRSVATVG PKMAMDVNAI VEGAQYLTAA VPNVPFVDEI TGEPQGLTA PIVHFGSVIS LWLLFALPVW SAAYKAAGAD TAEWVGVSQV TEDAPGIGLY GKYAPEYDGP TFREGLEYVL S FAWKPPIL ...String:
DHKRSRMMKS LALAAVGLAV GAEAFAPTPM VGGAKLALRT SSTRSVATVG PKMAMDVNAI VEGAQYLTAA VPNVPFVDEI TGEPQGLTA PIVHFGSVIS LWLLFALPVW SAAYKAAGAD TAEWVGVSQV TEDAPGIGLY GKYAPEYDGP TFREGLEYVL S FAWKPPIL IAWKPRADLD RAMMDPARDT VVSSLYKSLG GALDKTAVYD EEDQLLILSD METFPETELG RRRVAQAEAA GW FTGNPSF GKSLIEYSEE TKKGMREPGT VSISAKELAA LRAEAAKK

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Macromolecule #9: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 9 / Number of copies: 69 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #10: Chlorophyll c2

MacromoleculeName: Chlorophyll c2 / type: ligand / ID: 10 / Number of copies: 6 / Formula: KC2
Molecular weightTheoretical: 608.926 Da
Chemical component information

ChemComp-KC2:
Chlorophyll c2

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Macromolecule #11: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...

MacromoleculeName: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol
type: ligand / ID: 11 / Number of copies: 5 / Formula: IHT
Molecular weightTheoretical: 550.856 Da
Chemical component information

ChemComp-IHT:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol

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Macromolecule #12: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...

MacromoleculeName: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17- ...Name: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol
type: ligand / ID: 12 / Number of copies: 21 / Formula: II0
Molecular weightTheoretical: 564.84 Da
Chemical component information

ChemComp-II0:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol

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Macromolecule #13: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 13 / Number of copies: 4 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #14: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...

MacromoleculeName: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca- ...Name: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol
type: ligand / ID: 14 / Number of copies: 2 / Formula: II3
Molecular weightTheoretical: 566.856 Da
Chemical component information

ChemComp-II3:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol

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Macromolecule #15: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 15 / Number of copies: 2 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #16: (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta...

MacromoleculeName: (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene
type: ligand / ID: 16 / Number of copies: 2 / Formula: 8CT
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-8CT:
(6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene

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Macromolecule #17: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 17 / Number of copies: 1 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.84 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 610800
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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