+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of PSII-ACPII supercomplex from cryptophyte algae | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Photosystem II / PHOTOSYNTHESIS | |||||||||
Function / homology | ![]() thylakoid membrane / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / chlorophyll binding ...thylakoid membrane / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / photosynthetic electron transport in photosystem II / photosynthesis / respiratory electron transport chain / electron transfer activity / oxidoreductase activity / protein stabilization / iron ion binding / heme binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.47 Å | |||||||||
![]() | Li XY / Mao ZY / Shen JR / Han GY | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea. Authors: Zhiyuan Mao / Xingyue Li / Zhenhua Li / Liangliang Shen / Xiaoyi Li / Yanyan Yang / Wenda Wang / Tingyun Kuang / Jian-Ren Shen / Guangye Han / ![]() ![]() Abstract: Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin ...Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery. | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 257.2 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 48.6 KB 48.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 16.8 KB | Display | ![]() |
Images | ![]() | 74.8 KB | ||
Filedesc metadata | ![]() | 10.5 KB | ||
Others | ![]() ![]() | 475.4 MB 475.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8wb4MC ![]() 8xklC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #2
File | emd_37414_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_37414_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
+Entire : Structure of PSII-ACPII supercomplex from cryptophyte algae
+Supramolecule #1: Structure of PSII-ACPII supercomplex from cryptophyte algae
+Macromolecule #1: Photosystem II reaction center M
+Macromolecule #2: ACPII-1
+Macromolecule #3: ACPII-2
+Macromolecule #4: ACPII-3
+Macromolecule #5: ACPII-4
+Macromolecule #6: ACPII-5
+Macromolecule #7: ACPII-6
+Macromolecule #8: Photosystem II protein D1
+Macromolecule #9: Photosystem II D2 protein
+Macromolecule #10: Cytochrome b559 subunit alpha
+Macromolecule #11: Cytochrome b559 subunit beta
+Macromolecule #12: Photosystem II reaction center protein H
+Macromolecule #13: Photosystem II reaction center protein I
+Macromolecule #14: Photosystem II reaction center protein J
+Macromolecule #15: Photosystem II reaction center protein K
+Macromolecule #16: Photosystem II reaction center protein O
+Macromolecule #17: Photosystem II reaction center protein Q
+Macromolecule #18: Photosystem II reaction center protein R
+Macromolecule #19: CCPII-S
+Macromolecule #20: Photosystem II reaction center protein T
+Macromolecule #21: Photosystem II reaction center protein U
+Macromolecule #22: Photosystem II cytochrome c550
+Macromolecule #23: Photosystem II reaction center X protein
+Macromolecule #24: Photosystem II reaction center protein Ycf12
+Macromolecule #25: Photosystem II reaction center protein Z
+Macromolecule #26: Photosystem II CP47 reaction center protein
+Macromolecule #27: Photosystem II CP43 reaction center protein
+Macromolecule #28: Photosystem II reaction center protein G
+Macromolecule #29: Photosystem II reaction center protein L
+Macromolecule #30: Photosystem II reaction center protein W
+Macromolecule #31: (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta...
+Macromolecule #32: CHLOROPHYLL A
+Macromolecule #33: Chlorophyll c2
+Macromolecule #34: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #35: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #36: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #37: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #38: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #39: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #40: CA-MN4-O5 CLUSTER
+Macromolecule #41: PHEOPHYTIN A
+Macromolecule #42: CHLORIDE ION
+Macromolecule #43: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+Macromolecule #44: BICARBONATE ION
+Macromolecule #45: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #46: FE (II) ION
+Macromolecule #47: PROTOPORPHYRIN IX CONTAINING FE
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |