EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 4, Page 591-597, Year 2024
掲載日	2024年1月29日
著者	Yehudi Bloch / Jan Felix / Romain Merceron / Mathias Provost / Royan Alipour Symakani / Robin De Backer / Elisabeth Lambert / Ahmad R Mehdipour / Savvas N Savvides /
PubMed 要旨	Cell-surface receptor complexes mediated by pro-inflammatory interleukin (IL)-12 and IL-23, both validated therapeutic targets, are incompletely understood due to the lack of structural insights into ...Cell-surface receptor complexes mediated by pro-inflammatory interleukin (IL)-12 and IL-23, both validated therapeutic targets, are incompletely understood due to the lack of structural insights into their complete extracellular assemblies. Furthermore, there is a paucity of structural details describing the IL-12-receptor interaction interfaces, in contrast to IL-23-receptor complexes. Here we report structures of fully assembled mouse IL-12/human IL-23-receptor complexes comprising the complete extracellular segments of the cognate receptors determined by electron cryo-microscopy. The structures reveal key commonalities but also surprisingly diverse features. Most notably, whereas IL-12 and IL-23 both utilize a conspicuously presented aromatic residue on their α-subunit as a hotspot to interact with the N-terminal Ig domain of their high-affinity receptors, only IL-12 juxtaposes receptor domains proximal to the cell membrane. Collectively, our findings will help to complete our understanding of cytokine-mediated assemblies of tall cytokine receptors and will enable a cytokine-specific interrogation of IL-12/IL-23 signaling in physiology and disease.
リンク	Nat Struct Mol Biol / PubMed:38287195
手法	EM (単粒子) / X線回折
解像度	2 - 6.7 Å
構造データ	16820 8odz EMDB-16820, PDB-8odz: Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1). 手法: EM (単粒子) / 解像度: 3.6 Å 16821 8oe0 EMDB-16821, PDB-8oe0: Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 2). 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-16822: Cryo-EM structure of the murine IL-12 complete extracellular signaling complex (Class 1). 手法: EM (単粒子) / 解像度: 5.6 Å EMDB-16823: Cryo-EM structure of the murine IL-12 complete extracellular signaling complex (Class 2). 手法: EM (単粒子) / 解像度: 6.7 Å 16824 8oe4 EMDB-16824, PDB-8oe4: Cryo-EM structure of a pre-dimerized human IL-23 complete extracellular signaling complex. 手法: EM (単粒子) / 解像度: 3.6 Å 17580 8pb1 EMDB-17580, PDB-8pb1: Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1), obtained after local refinement. 手法: EM (単粒子) / 解像度: 3.5 Å PDB-8c7m: Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb fragment. 手法: X-RAY DIFFRACTION / 解像度: 2.56 Å PDB-8cr5: Murine Interleukin-12 receptor beta 1 domain 1 in complex with murine Interleukin-12 beta. 手法: X-RAY DIFFRACTION / 解像度: 2.15 Å PDB-8cr6: mouse Interleukin-12 手法: X-RAY DIFFRACTION / 解像度: 2.85 Å PDB-8cr8: human Interleukin-23 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å PDB-8odx: Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb4 fragment and VHH. 手法: X-RAY DIFFRACTION / 解像度: 4.4 Å PDB-8ppm: IL-12Rb1 neutralizing Fab4, crystal kappa variant 手法: X-RAY DIFFRACTION / 解像度: 2.03 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-CL: Unknown entry / クロリド ChemComp-HOH: WATER ChemComp-TB: TERBIUM(III) ION ChemComp-K: Unknown entry / カリウムカチオン
由来	mus musculus (ハツカネズミ) homo sapiens (ヒト) homo sapiens x mus musculus hybrid cell line (ヒト) lama glama (ラマ)
キーワード	CYTOKINE / receptor / antagonist / antibody / complex / inflammation / glycoprotein / immunity / heterodimer / EXTRACELLULAR / FIBRONECTIN TYPE III / fibronectin / SIGNALING PROTEIN / IMMUNOSUPPRESSANT / Fab fragment / designed crystal contact