EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.
ジャーナル・号・ページ	Mol Cell, Vol. 84, Issue 3, Page 463-475.e5, Year 2024
掲載日	2024年2月1日
著者	Chunyi Hu / Mason T Myers / Xufei Zhou / Zhonggang Hou / Macy L Lozen / Ki Hyun Nam / Yan Zhang / Ailong Ke /
PubMed 要旨	Type I CRISPR-Cas systems utilize the RNA-guided Cascade complex to identify matching DNA targets and the nuclease-helicase Cas3 to degrade them. Among the seven subtypes, type I-C is compact in size ...Type I CRISPR-Cas systems utilize the RNA-guided Cascade complex to identify matching DNA targets and the nuclease-helicase Cas3 to degrade them. Among the seven subtypes, type I-C is compact in size and highly active in creating large-sized genome deletions in human cells. Here, we use four cryoelectron microscopy snapshots to define its RNA-guided DNA binding and cleavage mechanisms in high resolution. The non-target DNA strand (NTS) is accommodated by I-C Cascade in a continuous binding groove along the juxtaposed Cas11 subunits. Binding of Cas3 further traps a flexible bulge in NTS, enabling NTS nicking. We identified two anti-CRISPR proteins AcrIC8 and AcrIC9 that strongly inhibit Neisseria lactamica I-C function. Structural analysis showed that AcrIC8 inhibits PAM recognition through allosteric inhibition, whereas AcrIC9 achieves so through direct competition. Both Acrs potently inhibit I-C-mediated genome editing and transcriptional modulation in human cells, providing the first off-switches for type I CRISPR eukaryotic genome engineering.
リンク	Mol Cell / PubMed:38242128 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 3.64 Å
構造データ	29877 8g9s EMDB-29877, PDB-8g9s: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.4 Å 29878 8g9t EMDB-29878, PDB-8g9t: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.6 Å 29879 8g9u EMDB-29879, PDB-8g9u: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.0 Å 29896 8gaf EMDB-29896, PDB-8gaf: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.64 Å 29900 8gam EMDB-29900, PDB-8gam: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.46 Å 29901 8gan EMDB-29901, PDB-8gan: Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications 手法: EM (単粒子) / 解像度: 3.26 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	neisseria lactamica (バクテリア) rhodobacter phage rcnl1 (ファージ)
キーワード	HYDROLASE/RNA / CRISPR / type I-C / cascade / anti-CRISPR / HYDROLASE-RNA complex / DNA BINDING PROTEIN / HYDROLASE/RNA/DNA / HYDROLASE-RNA-DNA complex