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-Structure paper
タイトル | Extracellular cytochrome nanowires appear to be ubiquitous in prokaryotes. |
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ジャーナル・号・ページ | Cell, Vol. 186, Issue 13, Page 2853-22864.e8, Year 2023 |
掲載日 | 2023年6月22日 |
著者 | Diana P Baquero / Virginija Cvirkaite-Krupovic / Shengen Shawn Hu / Jessie Lynda Fields / Xing Liu / Christopher Rensing / Edward H Egelman / Mart Krupovic / Fengbin Wang / |
PubMed 要旨 | Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to ...Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to numerous potential applications. However, whether other organisms employ similar ECNs for electron transfer remains unknown. Here, using cryoelectron microscopy, we describe the atomic structures of two ECNs from two major orders of hyperthermophilic archaea present in deep-sea hydrothermal vents and terrestrial hot springs. Homologs of Archaeoglobus veneficus ECN are widespread among mesophilic methane-oxidizing Methanoperedenaceae, alkane-degrading Syntrophoarchaeales archaea, and in the recently described megaplasmids called Borgs. The ECN protein subunits lack similarities in their folds; however, they share a common heme arrangement, suggesting an evolutionarily optimized heme packing for efficient electron transfer. The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range electron transfer in both prokaryotic domains of life. |
リンク | Cell / PubMed:37290436 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 3.8 - 3.9 Å |
構造データ | EMDB-27911, PDB-8e5f: EMDB-27912, PDB-8e5g: |
化合物 | ChemComp-HEC: |
由来 |
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キーワード | ELECTRON TRANSPORT / helical symmetry / cytochrome filmanet / conductive nanowires / microbial nanowires |