+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27912 | |||||||||
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Title | Cryo-EM of A. veneficus cytochrome filament | |||||||||
Map data | cryo-EM of A. veneficus cytochrome filament | |||||||||
Sample |
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Keywords | helical symmetry / cytochrome filmanet / conductive nanowires / microbial nanowires / ELECTRON TRANSPORT | |||||||||
Function / homology | Multiheme cytochrome superfamily / Uncharacterized protein Function and homology information | |||||||||
Biological species | Archaeoglobus veneficus (archaea) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
Authors | Wang F / Baquero DP / Krupovic M / Egelman EH | |||||||||
Funding support | United States, 2 items
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Citation | Journal: Cell / Year: 2023 Title: Extracellular cytochrome nanowires appear to be ubiquitous in prokaryotes. Authors: Diana P Baquero / Virginija Cvirkaite-Krupovic / Shengen Shawn Hu / Jessie Lynda Fields / Xing Liu / Christopher Rensing / Edward H Egelman / Mart Krupovic / Fengbin Wang / Abstract: Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to ...Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to numerous potential applications. However, whether other organisms employ similar ECNs for electron transfer remains unknown. Here, using cryoelectron microscopy, we describe the atomic structures of two ECNs from two major orders of hyperthermophilic archaea present in deep-sea hydrothermal vents and terrestrial hot springs. Homologs of Archaeoglobus veneficus ECN are widespread among mesophilic methane-oxidizing Methanoperedenaceae, alkane-degrading Syntrophoarchaeales archaea, and in the recently described megaplasmids called Borgs. The ECN protein subunits lack similarities in their folds; however, they share a common heme arrangement, suggesting an evolutionarily optimized heme packing for efficient electron transfer. The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range electron transfer in both prokaryotic domains of life. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_27912.map.gz | 6.3 MB | EMDB map data format | |
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Header (meta data) | emd-27912-v30.xml emd-27912.xml | 13.8 KB 13.8 KB | Display Display | EMDB header |
Images | emd_27912.png | 51.7 KB | ||
Others | emd_27912_half_map_1.map.gz emd_27912_half_map_2.map.gz | 200.7 MB 200.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27912 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27912 | HTTPS FTP |
-Validation report
Summary document | emd_27912_validation.pdf.gz | 949.9 KB | Display | EMDB validaton report |
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Full document | emd_27912_full_validation.pdf.gz | 949.5 KB | Display | |
Data in XML | emd_27912_validation.xml.gz | 15.8 KB | Display | |
Data in CIF | emd_27912_validation.cif.gz | 18.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27912 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27912 | HTTPS FTP |
-Related structure data
Related structure data | 8e5gMC 8e5fC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_27912.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | cryo-EM of A. veneficus cytochrome filament | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half B
File | emd_27912_half_map_1.map | ||||||||||||
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Annotation | half B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half A
File | emd_27912_half_map_2.map | ||||||||||||
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Annotation | half A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : extracellular cytochrome filament
Entire | Name: extracellular cytochrome filament |
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Components |
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-Supramolecule #1: extracellular cytochrome filament
Supramolecule | Name: extracellular cytochrome filament / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Archaeoglobus veneficus (archaea) |
-Macromolecule #1: c-type cytochrome
Macromolecule | Name: c-type cytochrome / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Archaeoglobus veneficus (archaea) |
Molecular weight | Theoretical: 27.958664 KDa |
Sequence | String: MNGGKLALLM VTLAAMGTLV LPSTTSLFLG QHMWYNISGT GNNLPCEKCH ADVFAEFKNN PGAHKTIGGG TDTVEHIRAA CGECHRTSV VGTFASGDGT SATPGQEAHA AATIACMACH EFGPNGNAPY SGAPVAGGFD NVTTDTASSP YNYDNGDTTY G TKEAHQTF ...String: MNGGKLALLM VTLAAMGTLV LPSTTSLFLG QHMWYNISGT GNNLPCEKCH ADVFAEFKNN PGAHKTIGGG TDTVEHIRAA CGECHRTSV VGTFASGDGT SATPGQEAHA AATIACMACH EFGPNGNAPY SGAPVAGGFD NVTTDTASSP YNYDNGDTTY G TKEAHQTF IERAVEDKTL IDSNEACIAC HTYVPVKINW THKVSLEFNC TYEYNTGTSG VTTHYNVTNW TVNGTRYTTV FG NTTGNGS VNDASNWPGW YPYSW UniProtKB: Uncharacterized protein |
-Macromolecule #2: HEME C
Macromolecule | Name: HEME C / type: ligand / ID: 2 / Number of copies: 4 / Formula: HEC |
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Molecular weight | Theoretical: 618.503 Da |
Chemical component information | ChemComp-HEC: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 6 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 32.74 Å Applied symmetry - Helical parameters - Δ&Phi: 33.52 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 92602 |
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Startup model | Type of model: NONE |
Final angle assignment | Type: NOT APPLICABLE |