Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Conformational changes in mitochondrial complex I of the thermophilic eukaryote .
Journal, issue, pages	Sci Adv, Vol. 8, Issue 47, Page eadc9952, Year 2022
Publish date	Nov 25, 2022
Authors	Eike Laube / Jakob Meier-Credo / Julian D Langer / Werner Kühlbrandt /
PubMed Abstract	Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. ...Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus , determined by cryoEM up to 2.4-Å resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping.
External links	Sci Adv / PubMed:36427319 / PubMed Central
Methods	EM (single particle)
Resolution	2.44 - 2.83 Å
Structure data	14791 7zm7 EMDB-14791, PDB-7zm7: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) Method: EM (single particle) / Resolution: 2.77 Å 14792 7zm8 EMDB-14792, PDB-7zm8: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) - membrane arm Method: EM (single particle) / Resolution: 2.76 Å 14794 7zmb EMDB-14794, PDB-7zmb: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) Method: EM (single particle) / Resolution: 2.75 Å 14796 7zme EMDB-14796, PDB-7zme: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) - membrane arm Method: EM (single particle) / Resolution: 2.83 Å 14797 7zmg EMDB-14797, PDB-7zmg: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) Method: EM (single particle) / Resolution: 2.44 Å 14798 7zmh EMDB-14798, PDB-7zmh: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm Method: EM (single particle) / Resolution: 2.47 Å
Chemicals	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM / Phosphatidylcholine ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-CDL: CARDIOLIPIN / phospholipidYM / Cardiolipin ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM / Phosphatidylethanolamine ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster ChemComp-SF4: IRON/SULFUR CLUSTER / Iron–sulfur cluster ChemComp-FMN: FLAVIN MONONUCLEOTIDE / Flavin mononucleotide ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate ChemComp-ZN: Unknown entry ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-HOH: WATER / Water ChemComp-LMN: Lauryl Maltose Neopentyl Glycol / detergent*YM
Source	chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
Keywords	OXIDOREDUCTASE / Proton transporter / Mitochondrial membrane protein / Complex