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TitleDe novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals.
Journal, issue, pagesNat Commun, Vol. 13, Page 4376-4376, Year 2022
Publish dateJan 26, 2022 (structure data deposition date)
AuthorsTetreau, G. / Sawaya, M.R. / De Zitter, E. / Andreeva, E.A. / Banneville, A.S. / Schibrowsky, N.A. / Coquelle, N. / Brewster, A.S. / Grunbein, M.L. / Kovacs, G.N. ...Tetreau, G. / Sawaya, M.R. / De Zitter, E. / Andreeva, E.A. / Banneville, A.S. / Schibrowsky, N.A. / Coquelle, N. / Brewster, A.S. / Grunbein, M.L. / Kovacs, G.N. / Hunter, M.S. / Kloos, M. / Sierra, R.G. / Schiro, G. / Qiao, P. / Stricker, M. / Bideshi, D. / Young, I.D. / Zala, N. / Engilberge, S. / Gorel, A. / Signor, L. / Teulon, J.M. / Hilpert, M. / Foucar, L. / Bielecki, J. / Bean, R. / de Wijn, R. / Sato, T. / Kirkwood, H. / Letrun, R. / Batyuk, A. / Snigireva, I. / Fenel, D. / Schubert, R. / Canfield, E.J. / Alba, M.M. / Laporte, F. / Despres, L. / Bacia, M. / Roux, A. / Chapelle, C. / Riobe, F. / Maury, O. / Ling, W.L. / Boutet, S. / Mancuso, A. / Gutsche, I. / Girard, E. / Barends, T.R.M. / Pellequer, J.L. / Park, H.W. / Laganowsky, A.D. / Rodriguez, J. / Burghammer, M. / Shoeman, R.L. / Doak, R.B. / Weik, M. / Sauter, N.K. / Federici, B. / Cascio, D. / Schlichting, I. / Colletier, J.P.
External linksNat Commun / PubMed:35902572
MethodsX-ray diffraction
Resolution2.4 - 3.4 Å
Structure data

PDB-7qx4:
mosquitocidal Cry11Aa determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-7qx5:
mosquitocidal Cry11Aa-Y449F determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-7qx6:
mosquitocidal Cry11Aa-E583Q determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-7qx7:
mosquitocidal Cry11Aa-F17Y determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

PDB-7qyd:
mosquitocidal Cry11Ba determined at pH 6.5 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7r1e:
Mosquitocidal Cry11Ba determined at pH 10.4 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • bacillus thuringiensis serovar israelensis (bacteria)
  • bacillus thuringiensis serovar jegathesan (bacteria)
KeywordsTOXIN / naturally-occurring crystals mosquitocidal toxin serial femtosecond crystallography

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