タイトル | Functional metagenomic screening identifies an unexpected beta-glucuronidase. |
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ジャーナル・号・ページ | Nat. Chem. Biol., Vol. 18, Page 1096-1103, Year 2022 |
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掲載日 | 2021年12月1日 (構造データの登録日) |
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著者 | Neun, S. / Brear, P. / Campbell, E. / Tryfona, T. / El Omari, K. / Wagner, A. / Dupree, P. / Hyvonen, M. / Hollfelder, F. |
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リンク | Nat. Chem. Biol. / PubMed:35799064 |
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手法 | X線回折 |
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解像度 | 1.95 - 2.41 Å |
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構造データ | PDB-7qe1: Crystal structure of apo SN243 手法: X-RAY DIFFRACTION / 解像度: 1.95 Å PDB-7qe2: Crystal structure of D-glucuronic acid bound to SN243 手法: X-RAY DIFFRACTION / 解像度: 2.15 Å PDB-7qea: Crystal structure of fluorescein-di-Beta-D-glucuronide bound to a mutant of SN243 (D415A) 手法: X-RAY DIFFRACTION / 解像度: 2.28 Å PDB-7qee: SN243 mutant D415N bound to para-nitrophenyl-Beta-D-glucuronide 手法: X-RAY DIFFRACTION / 解像度: 2.374 Å PDB-7qef: Crystal structure of para-nitrophenyl-Beta-D-glucuronide bound to a mutant of SN243 (D415A) 手法: X-RAY DIFFRACTION / 解像度: 2.41 Å PDB-7qg4: Apo crystal structure of a mutant of SN243 (D415N) 手法: X-RAY DIFFRACTION / 解像度: 2.08 Å |
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化合物 | ChemComp-B9I: (2~{S},3~{S},4~{S},5~{R},6~{S})-3,4,5-tris(oxidanyl)-6-[(1~{R})-6'-oxidanyl-3-oxidanylidene-spiro[2-benzofuran-1,9'-xanthene]-3'-yl]oxy-oxane-2-carboxylic acid
ChemComp-C3G: 4-nitrophenyl beta-D-glucopyranosiduronic acid
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由来 | - unidentified (未定義)
- synthetic construct (人工物)
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キーワード | HYDROLASE / enzyme discovery / carbohydrate-active enzymes (CAZy) / protein engineering / functional metagenomics) |
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