Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Ab initio phasing macromolecular structures using electron-counted MicroED data.
Journal, issue, pages	Nat Methods, Vol. 19, Issue 6, Page 724-729, Year 2022
Publish date	May 30, 2022
Authors	Michael W Martynowycz / Max T B Clabbers / Johan Hattne / Tamir Gonen /
PubMed Abstract	Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct electron detector in counting mode. Microcrystals ...Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct electron detector in counting mode. Microcrystals were identified using a scanning electron microscope (SEM) and thinned with a focused ion beam (FIB) to produce crystalline lamellae of ideal thickness. Continuous-rotation data were collected using an ultra-low exposure rate to enable electron counting in diffraction. For the first sample, triclinic lysozyme extending to a resolution of 0.87 Å, an ideal helical fragment of only three alanine residues provided initial phases. These phases were improved using density modification, allowing the entire atomic structure to be built automatically. A similar approach was successful on a second macromolecular sample, proteinase K, which is much larger and diffracted to a resolution of 1.5 Å. These results demonstrate that macromolecules can be determined to sub-ångström resolution by MicroED and that ab initio phasing can be successfully applied to counting data.
External links	Nat Methods / PubMed:35637302 / PubMed Central
Methods	EM (electron crystallography)
Resolution	0.87 - 1.5 Å
Structure data	25184 7skw EMDB-25184, PDB-7skw: Ab initio structure of triclinic lysozyme from electron-counted MicroED data Method: EM (electron crystallography) 25185 7skx EMDB-25185, PDB-7skx: Ab initio structure of proteinase K from electron-counted MicroED data Method: EM (electron crystallography)
Chemicals	ChemComp-NO3: NITRATE ION ChemComp-HOH: WATER ChemComp-I3C: 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid ChemComp-CA: Unknown entry
Source	gallus gallus (chicken) parengyodontium album (fungus)
Keywords	HYDROLASE