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TitleStructural characterization of the Myxococcus xanthus encapsulin and ferritin-like cargo system gives insight into its iron storage mechanism.
Journal, issue, pagesStructure, Vol. 30, Issue 4, Page 551-563.e4, Year 2022
Publish dateApr 7, 2022
AuthorsElif Eren / Bing Wang / Dennis C Winkler / Norman R Watts / Alasdair C Steven / Paul T Wingfield /
PubMed AbstractEncapsulins are bacterial organelle-like cages involved in various aspects of metabolism, especially protection from oxidative stress. They can serve as vehicles for a wide range of medical ...Encapsulins are bacterial organelle-like cages involved in various aspects of metabolism, especially protection from oxidative stress. They can serve as vehicles for a wide range of medical applications. Encapsulin shell proteins are structurally similar to HK97 bacteriophage capsid protein and their function depends on the encapsulated cargos. The Myxococcus xanthus encapsulin system comprises EncA and three cargos: EncB, EncC, and EncD. EncB and EncC are similar to bacterial ferritins that can oxidize Fe to less toxic Fe. We analyzed EncA, EncB, and EncC by cryo-EM and X-ray crystallography. Cryo-EM shows that EncA cages can have T = 3 and T = 1 symmetry and that EncA T = 1 has a unique protomer arrangement. Also, we define EncB and EncC binding sites on EncA. X-ray crystallography of EncB and EncC reveals conformational changes at the ferroxidase center and additional metal binding sites, suggesting a mechanism for Fe oxidation and storage within the encapsulin shell.
External linksStructure / PubMed:35150605 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.86 - 3.45 Å
Structure data

24814

7s20

EMDB-24814, PDB-7s20:
M. xanthus encapsulin shell protein EncA with T=3 symmetry
Method: EM (single particle) / Resolution: 3.4 Å

24815

7s21

EMDB-24815, PDB-7s21:
M. xanthus encapsulin shell protein EncA with T=1 symmetry
Method: EM (single particle) / Resolution: 3.4 Å

24816

7s2t

EMDB-24816, PDB-7s2t:
M. xanthus encapsulin EncA bound to EncB targeting peptide
Method: EM (single particle) / Resolution: 3.45 Å

24832

7s4q

EMDB-24832, PDB-7s4q:
M. xanthus encapsulin EncA bound to EncC targeting peptide
Method: EM (single particle) / Resolution: 3.12 Å

PDB-7s5c:
M. xanthus ferritin-like protein EncB
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-7s5k:
M. xanthus ferritin-like protein EncB
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7s8t:
M. xanthus ferritin-like protein EncC
Method: X-RAY DIFFRACTION / Resolution: 2.49 Å

Chemicals

ChemComp-FE:
Unknown entry / Iron

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • myxococcus xanthus (bacteria)
KeywordsVIRUS LIKE PARTICLE / nanocage / encapsulin / iron storage / bacterial nano-compartment / CYTOSOLIC PROTEIN / cargo protein / encapsulated ferritin / ferroxidase

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