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TitleStructure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
Journal, issue, pagesCell Discov, Vol. 8, Issue 1, Page 12, Year 2022
Publish dateFeb 11, 2022
AuthorsAojie Wang / Feng Zhou / Congcong Liu / Dongsheng Gao / Ruxi Qi / Yiheng Yin / Sheng Liu / Yuanzhu Gao / Lutang Fu / Yinhe Xia / Yawei Xu / Chuanqing Wang / Zheng Liu /
PubMed AbstractGetah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% ...Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV. The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion. Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2. We have identified numerous glycosylation and S-acylation sites in E1 and E2. The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion. The S-acylation sites might be involved in stabilizing the transmembrane assembly of E1 and E2. In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket. The cholesterol and phospholipid stabilize the hydrophobic pocket in the viral envelope membrane. The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
External linksCell Discov / PubMed:35149682 / PubMed Central
MethodsEM (single particle)
Resolution2.81 - 3.84 Å
Structure data

31533

7fd2

EMDB-31533, PDB-7fd2:
Cryo-EM structure of an alphavirus, Getah virus
Method: EM (single particle) / Resolution: 2.81 Å

EMDB-31614: Cryo-EM map of GETV at the 5-fold axis of symmetry
Method: EM (single particle) / Resolution: 3.84 Å

EMDB-31615: Cryo-EM map of three fold axis of GETV
Method: EM (single particle) / Resolution: 2.92 Å

EMDB-31617: Cryo-EM map of GETV 2-fold axis
Method: EM (single particle) / Resolution: 2.85 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-PCW:
1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DOPC, phospholipid*YM

ChemComp-STE:
STEARIC ACID / Stearic acid

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-CLR:
CHOLESTEROL / Cholesterol

Source
  • getah virus
KeywordsVIRUS / mature / Infective

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