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Structure paper

TitleThe human microbiome encodes resistance to the antidiabetic drug acarbose.
Journal, issue, pagesNature, Vol. 600, Page 110-115, Year 2021
Publish dateMar 26, 2020 (structure data deposition date)
AuthorsBalaich, J. / Estrella, M. / Wu, G. / Jeffrey, P.D. / Biswas, A. / Zhao, L. / Korennykh, A. / Donia, M.S.
External linksNature / PubMed:34819672
MethodsX-ray diffraction
Resolution2.44 - 3.102 Å
Structure data

PDB-6wb4:
Microbiome-derived Acarbose Kinase Mak1 Labeled with selenomethionine
Method: X-RAY DIFFRACTION / Resolution: 2.593 Å

PDB-6wb5:
Microbiome-derived Acarbose Kinase Mak1 as a Complex with Acarbose and AMP-PNP
Method: X-RAY DIFFRACTION / Resolution: 3.102 Å

PDB-6wb7:
Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MN:
Unknown entry

ChemComp-NA:
Unknown entry

Source
  • uncultured bacterium (environmental samples)
  • actinoplanes sp. (strain atcc 31044 / cbs 674.73 / se50/110) (bacteria)
KeywordsTRANSFERASE / CARBOHYDRATE KINASE / ACARBOSE / NUCLEOTIDE BINDING / ATP BINDING / METAL ION BINDING / RIBOKINASE ACTIVITY / COMPLEX

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