EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of polyamine transport by human ATP13A2 (PARK9).
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 22, Page 4635-44649.e8, Year 2021
掲載日	2021年11月18日
著者	Sue Im Sim / Sören von Bülow / Gerhard Hummer / Eunyong Park /
PubMed 要旨	Polyamines are small, organic polycations that are ubiquitous and essential to all forms of life. Currently, how polyamines are transported across membranes is not understood. Recent studies have ...Polyamines are small, organic polycations that are ubiquitous and essential to all forms of life. Currently, how polyamines are transported across membranes is not understood. Recent studies have suggested that ATP13A2 and its close homologs, collectively known as P5B-ATPases, are polyamine transporters at endo-/lysosomes. Loss-of-function mutations of ATP13A2 in humans cause hereditary early-onset Parkinson's disease. To understand the polyamine transport mechanism of ATP13A2, we determined high-resolution cryoelectron microscopy (cryo-EM) structures of human ATP13A2 in five distinct conformational intermediates, which together, represent a near-complete transport cycle of ATP13A2. The structural basis of the polyamine specificity was revealed by an endogenous polyamine molecule bound to a narrow, elongated cavity within the transmembrane domain. The structures show an atypical transport path for a water-soluble substrate, in which polyamines may exit within the cytosolic leaflet of the membrane. Our study provides important mechanistic insights into polyamine transport and a framework to understand the functions and mechanisms of P5B-ATPases.
リンク	Mol Cell / PubMed:34715013 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.7 Å
構造データ	EMDB-24212: Cryo-EM structure of ATP13A2 in the E2-Pi state 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-24213: Cryo-EM structure of ATP13A2 in the E2-Pi state 手法: EM (単粒子) / 解像度: 3.2 Å 24214 7n70 EMDB-24214, PDB-7n70: Cryo-EM structure of ATP13A2 in the BeF-bound E2P-like state 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-24215: Cryo-EM structure of ATP13A2 in the E2-Pi state 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-24216: Cryo-EM structure of ATP13A2 in the AlF-bound E2-Pi-like state 手法: EM (単粒子) / 解像度: 2.8 Å 24217 7n72 EMDB-24217, PDB-7n72: Cryo-EM structure of ATP13A2 in the AlF-bound E2-Pi-like state 手法: EM (単粒子) / 解像度: 2.5 Å 24218 7n73 EMDB-24218, PDB-7n73: Cryo-EM structure of ATP13A2 in the ADP-AlF-bound E1P-ADP-like state 手法: EM (単粒子) / 解像度: 2.9 Å 24219 7n74 EMDB-24219, PDB-7n74: Cryo-EM structure of ATP13A2 D508N mutant in the E1-ATP-like state 手法: EM (単粒子) / 解像度: 2.8 Å 24220 7n75 EMDB-24220, PDB-7n75: Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 1 手法: EM (単粒子) / 解像度: 2.9 Å 24221 7n76 EMDB-24221, PDB-7n76: Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 2 手法: EM (単粒子) / 解像度: 2.9 Å 24222 7n77 EMDB-24222, PDB-7n77: Cryo-EM structure of ATP13A2 D458N/D962N mutant in the AlF-bound E1P-like state 手法: EM (単粒子) / 解像度: 3.2 Å 24223 7n78 EMDB-24223, PDB-7n78: Cryo-EM structure of ATP13A2 in the E2-Pi state 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-SPM: SPERMINE / スペルミン / スペルミン ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-HOH: WATER / 水 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / P-type ATPase / P5B-ATPase / polyamine transporter