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Structure paper

TitleStructure of respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateJul 26, 2021
AuthorsPiotr Kolata / Rouslan G Efremov /
PubMed AbstractRespiratory complex I is a multi-subunit membrane protein complex that reversibly couples NADH oxidation and ubiquinone reduction with proton translocation against transmembrane potential. Complex I ...Respiratory complex I is a multi-subunit membrane protein complex that reversibly couples NADH oxidation and ubiquinone reduction with proton translocation against transmembrane potential. Complex I from is among the best functionally characterized complexes, but its structure remains unknown, hindering further studies to understand the enzyme coupling mechanism. Here, we describe the single particle cryo-electron microscopy (cryo-EM) structure of the entire catalytically active complex I reconstituted into lipid nanodiscs. The structure of this mesophilic bacterial complex I displays highly dynamic connection between the peripheral and membrane domains. The peripheral domain assembly is stabilized by unique terminal extensions and an insertion loop. The membrane domain structure reveals novel dynamic features. Unusual conformation of the conserved interface between the peripheral and membrane domains suggests an uncoupled conformation of the complex. Considering constraints imposed by the structural data, we suggest a new simple hypothetical coupling mechanism for the molecular machine.
External linksElife / PubMed:34308841 / PubMed Central
MethodsEM (single particle)
Resolution2.1 - 6.7 Å
Structure data

12652

7nyh

EMDB-12652, PDB-7nyh:
Respiratory complex I from Escherichia coli - focused refinement of membrane arm
Method: EM (single particle) / Resolution: 3.6 Å

12653

7nyr

EMDB-12653, PDB-7nyr:
Respiratory complex I from Escherichia coli - conformation 1
Method: EM (single particle) / Resolution: 3.3 Å

12654

7nyu

EMDB-12654, PDB-7nyu:
Respiratory complex I from Escherichia coli - conformation 2
Method: EM (single particle) / Resolution: 3.8 Å

12655

7nyv

EMDB-12655, PDB-7nyv:
Respiratory complex I from Escherichia coli - conformation 3
Method: EM (single particle) / Resolution: 3.7 Å

12661

7nz1

EMDB-12661, PDB-7nz1:
Respiratory complex I from Escherichia coli - focused refinement of cytoplasmic arm
Method: EM (single particle) / Resolution: 2.1 Å

EMDB-13291:
Respiratory complex I from Escherichia coli solubilized in LMNG
Method: EM (single particle) / Resolution: 6.7 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli b (bacteria)
KeywordsELECTRON TRANSPORT / NADH:ubiquinone reductase (H+-translocating) / oxidative phosphorylation

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