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-Structure paper
タイトル | Cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with lipopolysaccharide. |
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ジャーナル・号・ページ | Biochem Biophys Res Commun, Vol. 571, Page 20-25, Year 2021 |
掲載日 | 2021年9月24日 |
著者 | Qingshan Luo / Huigang Shi / Xueqing Xu / |
PubMed 要旨 | Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven ...Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven lipopolysaccharide transport proteins (LptA-G). LptBFG, an type VI ATP-binding cassette (ABC) transporter, forms a stable complex with LptC, extracts LPS from the IM and powers LPS transport to the OM. Here we report the cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with LPS. The KpLptBFG-LPS structure provides detailed interactions between LPS and the transporter, while the KpLptBFGC-LPS structure may represent an intermediate state that the transmembrane helix of LptC has not been fully inserted into the transmembrane domains of LptBFG. |
リンク | Biochem Biophys Res Commun / PubMed:34303191 |
手法 | EM (単粒子) |
解像度 | 3.85 Å |
構造データ | EMDB-31087: EMDB-31088, PDB-7efo: |
化合物 | ChemComp-JSG: |
由来 |
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キーワード | MEMBRANE PROTEIN / ABC transporter |