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Structure paper

TitleThe final step of 40S ribosomal subunit maturation is controlled by a dual key lock.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateApr 28, 2021
AuthorsLaura Plassart / Ramtin Shayan / Christian Montellese / Dana Rinaldi / Natacha Larburu / Carole Pichereaux / Carine Froment / Simon Lebaron / Marie-Françoise O'Donohue / Ulrike Kutay / Julien Marcoux / Pierre-Emmanuel Gleizes / Celia Plisson-Chastang /
PubMed AbstractPreventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, ...Preventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3' end, is safeguarded by the protein DIM2, which both interacts with the endoribonuclease NOB1 and masks the rRNA cleavage site. To elucidate the control mechanism that unlocks NOB1 activity, we performed cryo-electron microscopy analysis of late human pre-40S particles purified using a catalytically inactive form of the ATPase RIO1. These structures, together with in vivo and in vitro functional analyses, support a model in which ATP-loaded RIO1 cooperates with ribosomal protein RPS26/eS26 to displace DIM2 from the 18S rRNA 3' end, thereby triggering final cleavage by NOB1; release of ADP then leads to RIO1 dissociation from the 40S subunit. This dual key lock mechanism requiring RIO1 and RPS26 guarantees the precise timing of pre-40S particle conversion into translation-competent ribosomal subunits.
External linksElife / PubMed:33908345 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.3 Å
Structure data

11440

6zuo

EMDB-11440, PDB-6zuo:
Human RIO1(kd)-StHA late pre-40S particle, structural state A (pre 18S rRNA cleavage)
Method: EM (single particle) / Resolution: 3.1 Å

11441

6zv6

EMDB-11441, PDB-6zv6:
Human RIO1(kd)-StHA late pre-40S particle, structural state B (post 18S rRNA cleavage)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-11442:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural State B, Body
Method: EM (single particle) / Resolution: 2.98 Å

EMDB-11443:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural State B, Head
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-11444:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural state B, platform
Method: EM (single particle) / Resolution: 2.98 Å

EMDB-11445:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural state A, body
Method: EM (single particle) / Resolution: 3.14 Å

EMDB-11446:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural state A, head
Method: EM (single particle) / Resolution: 3.17 Å

EMDB-11447:
Human pre-40S particle purified using RIO1(kd)-StHA as bait - Structural state A, platform
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / Human ribosome biogenesis / small ribosomal subunit / rRNA processing

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