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Structure paper

TitleThe molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.
Journal, issue, pagesJ. Biol. Chem., Vol. 296, Page 100662-100662, Year 2021
Publish dateApr 30, 2020 (structure data deposition date)
AuthorsRollen, K. / Granzin, J. / Remeeva, A. / Davari, M.D. / Gensch, T. / Nazarenko, V.V. / Kovalev, K. / Bogorodskiy, A. / Borshchevskiy, V. / Hemmer, S. ...Rollen, K. / Granzin, J. / Remeeva, A. / Davari, M.D. / Gensch, T. / Nazarenko, V.V. / Kovalev, K. / Bogorodskiy, A. / Borshchevskiy, V. / Hemmer, S. / Schwaneberg, U. / Gordeliy, V. / Jaeger, K.E. / Batra-Safferling, R. / Gushchin, I. / Krauss, U.
External linksJ. Biol. Chem. / PubMed:33862085
MethodsX-ray diffraction
Resolution1.36 - 1.9 Å
Structure data

PDB-6yx4:
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant with morpholine
Method: X-RAY DIFFRACTION / Resolution: 1.36 Å

PDB-6yx6:
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (no morpholine)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6yxb:
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (space group P21)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-7ab6:
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) I52T variant
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7ab7:
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) I52T Q148K variant
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7aby:
Crystal structure of iLOV-Q489K mutant
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-6LR:
morpholine

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

Source
  • chloroflexus aggregans (strain md-66 / dsm 9485) (bacteria)
  • arabidopsis thaliana (thale cress)
KeywordsFLUORESCENT PROTEIN / LOV domain / SIGNALING PROTEIN / BLUE LIGHT PHOTORECEPTOR / FLAVOPROTEIN

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