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Structure paper

TitleStructures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
Journal, issue, pagesMol Cell, Vol. 80, Issue 2, Page 227-236.e5, Year 2020
Publish dateOct 15, 2020
AuthorsStephanie Oerum / Tom Dendooven / Marjorie Catala / Laetitia Gilet / Clément Dégut / Aude Trinquier / Maxime Bourguet / Pierre Barraud / Sarah Cianferani / Ben F Luisi / Ciarán Condon / Carine Tisné /
PubMed AbstractThe pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ...The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 Å resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing.
External linksMol Cell / PubMed:32991829 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.3 - 3.07 Å
Structure data

10535

6tnn

EMDB-10535, PDB-6tnn:
Mini-RNase III (Mini-III) bound to 50S ribosome with precursor 23S rRNA
Method: EM (single particle) / Resolution: 3.07 Å

10543

6tpq

EMDB-10543, PDB-6tpq:
RNase M5 bound to 50S ribosome with precursor 5S rRNA
Method: EM (single particle) / Resolution: 3.07 Å

PDB-6tg6:
Toprim domain of RNase M5
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-6tgj:
RNA-binding domain of RNase M5
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • bacillus subtilis subsp. subtilis str. 168 (bacteria)
  • Bacillus subtilis subsp. subtilis str. 168
  • Bacillus subtilis subsp. subtilis str. 168
  • geobacillus stearothermophilus (bacteria)
KeywordsHYDROLASE / RNase M5 / RNase / rRNA / 5S rRNA / precursor rRNA / pre-5S rRNA / ribosomal RNA / ribonuclease / catalytic domain / toprim domain / RNA BINDING PROTEIN / novel fold / RNA-binding domain / RIBOSOMAL PROTEIN / Mini-RNase III / Mini-III / complex / ribosome / 50S / RNA maturation / RNA processing / precursor 23S rRNA / pre-23S rRNA / 23S rRNA / L3 / RNase III-like fold / M5 / precursor 5S rRNA / L18

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