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TitleThe molecular basis of tight nuclear tethering and inactivation of cGAS.
Journal, issue, pagesNature, Vol. 587, Issue 7835, Page 673-677, Year 2020
Publish dateSep 10, 2020
AuthorsBaoyu Zhao / Pengbiao Xu / Chesley M Rowlett / Tao Jing / Omkar Shinde / Yuanjiu Lei / A Phillip West / Wenshe Ray Liu / Pingwei Li /
PubMed AbstractNucleic acids derived from pathogens induce potent innate immune responses. Cyclic GMP-AMP synthase (cGAS) is a double-stranded DNA sensor that catalyses the synthesis of the cyclic dinucleotide ...Nucleic acids derived from pathogens induce potent innate immune responses. Cyclic GMP-AMP synthase (cGAS) is a double-stranded DNA sensor that catalyses the synthesis of the cyclic dinucleotide cyclic GMP-AMP, which mediates the induction of type I interferons through the STING-TBK1-IRF3 signalling axis. cGAS was previously thought to not react with self DNA owing to its cytosolic localization; however, recent studies have shown that cGAS is localized mostly in the nucleus and has low activity as a result of tight nuclear tethering. Here we show that cGAS binds to nucleosomes with nanomolar affinity and that nucleosome binding potently inhibits its catalytic activity. To elucidate the molecular basis of cGAS inactivation by nuclear tethering, we determined the structure of mouse cGAS bound to human nucleosome by cryo-electron microscopy. The structure shows that cGAS binds to a negatively charged acidic patch formed by histones H2A and H2B via its second DNA-binding site. High-affinity nucleosome binding blocks double-stranded DNA binding and maintains cGAS in an inactive conformation. Mutations of cGAS that disrupt nucleosome binding alter cGAS-mediated signalling in cells.
External linksNature / PubMed:32911481 / PubMed Central
MethodsEM (single particle)
Resolution2.98 - 6.8 Å
Structure data

22046

6x59

EMDB-22046, PDB-6x59:
The mouse cGAS catalytic domain binding to human assembled nucleosome
Method: EM (single particle) / Resolution: 2.98 Å

22047

6x5a

EMDB-22047, PDB-6x5a:
The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells
Method: EM (single particle) / Resolution: 4.36 Å

22206

6xjd

EMDB-22206, PDB-6xjd:
Two mouse cGAS catalytic domain binding to human assembled nucleosome
Method: EM (single particle) / Resolution: 6.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsDNA BINDING PROTEIN/DNA/TRANSFERASE / Immunity / DNA BINDING PROTEIN-DNA-TRANSFERASE complex / IMMUNE SYSTEM/DNA / IMMUNE SYSTEM / IMMUNE SYSTEM-DNA complex

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