EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 4331, Year 2020
掲載日	2020年8月28日
著者	Jonathan A Flores / Bassam G Haddad / Kimberly A Dolan / Janette B Myers / Craig C Yoshioka / Jeremy Copperman / Daniel M Zuckerman / Steve L Reichow /
PubMed 要旨	Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel ...Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel activity of gap junctions; however, the molecular basis for these effects remains unknown. Here, we incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual lipid nanodisc system, mimicking a native cell-to-cell junction. Structural characterization by CryoEM reveals a lipid-induced stabilization to the channel, resulting in a 3D reconstruction at 1.9 Å resolution. Together with all-atom molecular dynamics simulations, it is shown that Cx46/50 in turn imparts long-range stabilization to the dynamic local lipid environment that is specific to the extracellular lipid leaflet. In addition, ~400 water molecules are resolved in the CryoEM map, localized throughout the intercellular permeation pathway and contributing to the channel architecture. These results illustrate how the aqueous-lipid environment is integrated with the architectural stability, structure and function of gap junction communication channels.
リンク	Nat Commun / PubMed:32859914 / PubMed Central
手法	EM (単粒子)
解像度	1.9 - 2.5 Å
構造データ	22358 7jjp 7jkc EMDB-22358: Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 angstroms PDB-7jjp: Sheep Connexin-50 at 1.9 angstroms resolution by CryoEM PDB-7jkc: Sheep Connexin-46 at 1.9 angstroms resolution by CryoEM 手法: EM (単粒子) / 解像度: 1.94 Å 22382 7jlw 7jmd EMDB-22382, PDB-7jlw: Sheep Connexin-50 at 2.5 angstroms resolution, Lipid Class 1 PDB-7jmd: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 1 手法: EM (単粒子) / 解像度: 2.5 Å 22390 7jm9 7jn0 EMDB-22390, PDB-7jm9: Sheep Connexin-50 at 2.5 angstroms reoslution, Lipid Class 2 PDB-7jn0: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 2 手法: EM (単粒子) / 解像度: 2.5 Å 22391 7jmc 7jn1 EMDB-22391, PDB-7jmc: Sheep Connexin-50 at 2.5 angstroms resolution, Lipid Class 3 PDB-7jn1: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 3 手法: EM (単粒子) / 解像度: 2.5 Å
化合物	ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-HOH*: WATER
由来	ovis aries (ヒツジ) Sheep (ヒツジ)
キーワード	MEMBRANE PROTEIN / Connexin / Gap Junction / Nanodisc / Lipid