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TitleStructure comparison of the chimeric AAV2.7m8 vector with parental AAV2.
Journal, issue, pagesJ Struct Biol, Vol. 209, Issue 2, Page 107433, Year 2020
Publish dateFeb 1, 2020
AuthorsAntonette Bennett / Annahita Keravala / Victoria Makal / Justin Kurian / Brahim Belbellaa / Rangoli Aeran / Yu-Shan Tseng / Duncan Sousa / John Spear / Mehdi Gasmi / Mavis Agbandje-McKenna /
PubMed AbstractThe AAV2.7m8 vector is an engineered capsid with a 10-amino acid insertion in adeno-associated virus (AAV) surface variable region VIII (VR-VIII) resulting in the alteration of an antigenic region of ...The AAV2.7m8 vector is an engineered capsid with a 10-amino acid insertion in adeno-associated virus (AAV) surface variable region VIII (VR-VIII) resulting in the alteration of an antigenic region of AAV2 and the ability to efficiently transduce retina cells following intravitreal administration. Directed evolution and in vivo screening in the mouse retina isolated this vector. In the present study, we sought to identify the structural differences between a recombinant AAV2.7m8 (rAAV2.7m8) vector packaging a GFP genome and its parental serotype, AAV2, by cryo-electron microscopy (cryo-EM) and image reconstruction. The structures of rAAV2.7m8 and AAV2 were determined to 2.91 and 3.02 Å resolution, respectively. The rAAV2.7m8 amino acid side-chains for residues 219-745 (the last C-terminal residue) were interpretable in the density map with the exception of the 10 inserted amino acids. While observable in a low sigma threshold density, side-chains were only resolved at the base of the insertion, likely due to flexibility at the top of the loop. A comparison to parental AAV2 (ordered from residues 217-735) showed the structures to be similar, except at some side-chains that had different orientations and, in VR-VIII containing the 10 amino acid insertion. VR-VIII is part of an AAV2 antigenic epitope, and the difference is consistent with rAAV2.7m8's escape from a known AAV2 monoclonal antibody, C37-B. The observations provide valuable insight into the configuration of inserted surface peptides on the AAV capsid and structural differences to be leveraged for future AAV vector rational design, especially for retargeted tropism and antibody escape.
External linksJ Struct Biol / PubMed:31859208 / PubMed Central
MethodsEM (single particle)
Resolution2.91 - 3.02 Å
Structure data

20609

6u0r

EMDB-20609, PDB-6u0r:
Cryo-EM structure of the chimeric vector AAV2.7m8
Method: EM (single particle) / Resolution: 2.91 Å

20610

6u0v

EMDB-20610, PDB-6u0v:
Atomic-Resolution Cryo-EM Structure of AAV2 VLP
Method: EM (single particle) / Resolution: 3.02 Å

Chemicals

ChemComp-D5M:
2'-DEOXYADENOSINE-5'-MONOPHOSPHATE

Source
  • adeno-associated virus - 2
KeywordsVIRUS / Protrusion / insertion / receptor / VIRUS LIKE PARTICLE / axes / pore

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