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-Structure paper
Title | Structure of a mitochondrial ATP synthase with bound native cardiolipin. |
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Journal, issue, pages | Elife, Vol. 8, Year 2019 |
Publish date | Nov 18, 2019 |
Authors | Alexander Mühleip / Sarah E McComas / Alexey Amunts / |
PubMed Abstract | The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum ...The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum Euglenozoa that also includes human parasites. It features 29 different subunits, 8 of which are newly identified. The membrane region was determined to 2.8 Å resolution, enabling the identification of 37 associated lipids, including 25 cardiolipins, which provides insight into protein-lipid interactions and their functional roles. The rotor-stator interface comprises four membrane-embedded horizontal helices, including a distinct subunit . The dimer interface is formed entirely by phylum-specific components, and a peripherally associated subcomplex contributes to the membrane curvature. The central and peripheral stalks directly interact with each other. Last, the ATPase inhibitory factor 1 (IF) binds in a mode that is different from human, but conserved in Trypanosomatids. |
External links | Elife / PubMed:31738165 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.8 - 4.32 Å |
Structure data | EMDB-10467, PDB-6tdu: EMDB-10468, PDB-6tdv: EMDB-10469, PDB-6tdw: EMDB-10470, PDB-6tdx: EMDB-10471: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring in rotational state 1 EMDB-10472: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring rotational state 2 EMDB-10473, PDB-6te0: EMDB-10474: EMDB-10475: |
Chemicals | ChemComp-CDL: ChemComp-LMT: ChemComp-LPP: ChemComp-TRT: ChemComp-ATP: ChemComp-MG: ChemComp-ADP: |
Source |
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Keywords | MEMBRANE PROTEIN / mitochondria / ATP synthase |