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Structure paper

TitleCrystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping.
Journal, issue, pagesNucleic Acids Res., Vol. 47, Page 11943-11955, Year 2019
Publish dateMar 26, 2019 (structure data deposition date)
AuthorsSlyvka, A. / Zagorskaite, E. / Czapinska, H. / Sasnauskas, G. / Bochtler, M.
External linksNucleic Acids Res. / PubMed:31724709
MethodsX-ray diffraction
Resolution2.07 - 2.64 Å
Structure data

PDB-6r64:
N-terminal domain of modification dependent EcoKMcrA restriction endonuclease (NEco) in complex with C5mCGG target sequence
Method: X-RAY DIFFRACTION / Resolution: 2.64 Å

PDB-6t21:
N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5mCGA target sequence
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-6t22:
N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5hmCGA target sequence
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • escherichia coli k12 (bacteria)
  • synthetic construct (others)
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli k-12 (bacteria)
KeywordsHYDROLASE / EcoKMcrA / NEco / N-TERMINAL DOMAIN / MODIFICATION DEPENDENT RESTRICTION / 5-METHYLCYTOSINE / 5MC / 5-HYDROXYMETHYLCYTOSINE / 5HMC / HNH ENDONUCLEASE / BBA-ME NUCLEASE / ScoMcrA

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