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Structure paper

TitleHow a circularized tmRNA moves through the ribosome.
Journal, issue, pagesScience, Vol. 363, Issue 6428, Page 740-744, Year 2019
Publish dateFeb 15, 2019
AuthorsChristopher D Rae / Yuliya Gordiyenko / V Ramakrishnan /
PubMed AbstractDuring trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo- ...During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.
External linksScience / PubMed:30765567 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 4.3 Å
Structure data

4475

6q95

EMDB-4475, PDB-6q95:
Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome
Method: EM (single particle) / Resolution: 3.7 Å

4476

6q97

EMDB-4476, PDB-6q97:
Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome
Method: EM (single particle) / Resolution: 3.9 Å

4477

6q98

EMDB-4477, PDB-6q98:
Structure of tmRNA SmpB bound in P site of E. coli 70S ribosome
Method: EM (single particle) / Resolution: 4.3 Å

4478

6q9a

EMDB-4478, PDB-6q9a:
Structure of tmRNA SmpB bound past E site of E. coli 70S ribosome
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • escherichia coli (E. coli)
  • Thermus thermophilus HB27 (bacteria)
  • thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039) (bacteria)
KeywordsRIBOSOME / tmRNA SmpB trans-translation translation

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