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TitleAn Assembly Intermediate Structure of Rice Dwarf Virus Reveals a Hierarchical Outer Capsid Shell Assembly Mechanism.
Journal, issue, pagesStructure, Vol. 27, Issue 3, Page 439-448.e3, Year 2019
Publish dateMar 5, 2019
AuthorsYusuke Nakamichi / Naoyuki Miyazaki / Kenta Tsutsumi / Akifumi Higashiura / Hirotaka Narita / Kazuyoshi Murata / Atsushi Nakagawa /
PubMed AbstractNearly all viruses of the Reoviridae family possess a multi-layered capsid consisting of an inner layer with icosahedral T = 1 symmetry and a second-outer layer (composed of 260 copies of a trimeric ...Nearly all viruses of the Reoviridae family possess a multi-layered capsid consisting of an inner layer with icosahedral T = 1 symmetry and a second-outer layer (composed of 260 copies of a trimeric protein) exhibiting icosahedral T = 13 symmetry. Here we describe the construction and structural evaluation of an assembly intermediate of the Rice dwarf virus of the family Reoviridae stalled at the second capsid layer via targeted disruption of the trimer-trimer interaction interface in the second-layer capsid protein. Structural determination was performed by conventional and Zernike/Volta phase-contrast cryoelectron microscopy. The assembly defect second-layer capsid trimers bound exclusively to the outer surface of the innermost capsid layer at the icosahedral 3-fold axis. Furthermore, the second-layer assembly could not proceed without specific inter-trimer interactions. Our results suggest that the correct assembly pathway for second-layer capsid formation is highly controlled at the inter-layer and inter-trimer interactions.
External linksStructure / PubMed:30581044
MethodsEM (single particle)
Resolution6.3 - 13.43 Å
Structure data

EMDB-6712:
Inner capsid of Rice Dwarf Virus
Method: EM (single particle) / Resolution: 13.43 Å

EMDB-6713:
Inner capsid of Rice Dwarf Virus with T-trimers
Method: EM (single particle) / Resolution: 13.43 Å

EMDB-6989:
Inner capsid of Rice Dwarf Virus with T-trimers (Full particle)
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-6990:
Inner capsid of Rice Dwarf Virus with T-trimers (empty particle)
Method: EM (single particle) / Resolution: 6.3 Å

Source
  • Rice dwarf virus (isolate O)

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