EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α.
ジャーナル・号・ページ	Structure, Vol. 26, Issue 12, Page 1612-11625.e4, Year 2018
掲載日	2018年12月4日
著者	Kai-En Chen / Vikas A Tillu / Mintu Chandra / Brett M Collins /
PubMed 要旨	Phosphorylation of phosphoinositides by the class II phosphatidylinositol 3-kinase (PI3K) PI3K-C2α is essential for many processes, including neuroexocytosis and formation of clathrin-coated ...Phosphorylation of phosphoinositides by the class II phosphatidylinositol 3-kinase (PI3K) PI3K-C2α is essential for many processes, including neuroexocytosis and formation of clathrin-coated vesicles. A defining feature of the class II PI3Ks is a C-terminal module composed of phox-homology (PX) and C2 membrane interacting domains; however, the mechanisms that control their specific cellular localization remain poorly understood. Here we report the crystal structure of the C2 domain of PI3K-C2α in complex with the phosphoinositide head-group mimic inositol hexaphosphate, revealing two distinct pockets for membrane binding. The C2 domain preferentially binds to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate, and low-resolution structures of the combined PX-C2 module by small-angle X-ray scattering reveal a compact conformation in which cooperative lipid binding by each domain binding can occur. Finally, we demonstrate an unexpected role for calcium in perturbing the membrane interactions of the PX-C2 module, which we speculate may be important for regulating the activity of PI3K-C2α.
リンク	Structure / PubMed:30293811
手法	SAS (X-ray synchrotron) / X線回折
解像度	1.678 - 2.604 Å
構造データ	SASDD66: Phox homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) 手法: SAXS/SANS SASDD76: Phox Homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) in complex with inositol-hexaphosphate (IP6) 手法: SAXS/SANS PDB-6bty: Crystal structure of the PI3KC2alpha C2 domain in space group P41212 手法: X-RAY DIFFRACTION / 解像度: 1.678 Å PDB-6btz: Crystal structure of the PI3KC2alpha C2 domain in space group C121 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-6bu0: Crystal structure of the PI3KC2alpha C2 domain in complex with IP6 手法: X-RAY DIFFRACTION / 解像度: 2.427 Å PDB-6bub: Crystal structure of the PI3KC2alpha PX domain in space group P432 手法: X-RAY DIFFRACTION / 解像度: 2.604 Å
化合物	ChemComp-O4B: 1,4,7,10,13,16-HEXAOXACYCLOOCTADECANE / 18-クラウン-6 ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-FMT: FORMIC ACID / ギ酸 ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸
由来	homo sapiens (ヒト)
キーワード	TRANSFERASE / C2 domain / lipid binding / phosphoinositide / PI3-kinase / PX domain