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TitlePostcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.
Journal, issue, pagesScience, Vol. 358, Issue 6368, Page 1283-1288, Year 2017
Publish dateDec 8, 2017
AuthorsMax E Wilkinson / Sebastian M Fica / Wojciech P Galej / Christine M Norman / Andrew J Newman / Kiyoshi Nagai /
PubMed AbstractIntrons are removed from eukaryotic messenger RNA precursors by the spliceosome in two transesterification reactions-branching and exon ligation. The mechanism of 3'-splice site recognition during ...Introns are removed from eukaryotic messenger RNA precursors by the spliceosome in two transesterification reactions-branching and exon ligation. The mechanism of 3'-splice site recognition during exon ligation has remained unclear. Here we present the 3.7-angstrom cryo-electron microscopy structure of the yeast P-complex spliceosome immediately after exon ligation. The 3'-splice site AG dinucleotide is recognized through non-Watson-Crick pairing with the 5' splice site and the branch-point adenosine. After the branching reaction, protein factors work together to remodel the spliceosome and stabilize a conformation competent for 3'-splice site docking, thereby promoting exon ligation. The structure accounts for the strict conservation of the GU and AG dinucleotides at the 5' and 3' ends of introns and provides insight into the catalytic mechanism of exon ligation.
External linksScience / PubMed:29146871 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 4.0 Å
Structure data

3979

6exn

EMDB-3979, PDB-6exn:
Post-catalytic P complex spliceosome with 3' splice site docked
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-3980:
Post-catalytic P complex spliceosome with 3' splice site undocked
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsSPLICING / Spliceosome / P-complex / Exon ligation

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