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Structure paper

TitleStructure and assembly of the Ebola virus nucleocapsid.
Journal, issue, pagesNature, Vol. 551, Issue 7680, Page 394-397, Year 2017
Publish dateNov 16, 2017
AuthorsWilliam Wan / Larissa Kolesnikova / Mairi Clarke / Alexander Koehler / Takeshi Noda / Stephan Becker / John A G Briggs /
PubMed AbstractEbola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles ...Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein-nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment.
External linksNature / PubMed:29144446 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution6.6 - 9.1 Å
Structure data

3869

6ehl

EMDB-3869: The structure of Ebola virus nucleoprotein (residues 1-450) in nucleocapsid-like assemblies
PDB-6ehl: Model of the Ebola virus nucleoprotein in recombinant nucleocapsid-like assemblies
Method: EM (subtomogram averaging) / Resolution: 6.6 Å

EMDB-3870:
Cryo-electron tomogram of Ebola virus nucleoprotein, residues 1-450
Method: EM (tomography)

3871

6ehm

EMDB-3871: The structure of Ebola virus nucleocapsid-like assemblies from recombinant virus-like particles (nucleoprotein, VP24,VP35,VP40)
PDB-6ehm: Model of the Ebola virus nucleocapsid subunit from recombinant virus-like particles
Method: EM (subtomogram averaging) / Resolution: 7.3 Å

EMDB-3872:
Cryo-electron tomogram of Ebola virus virus-like particles
Method: EM (tomography)

EMDB-3873:
The structure of Ebola virus nucleocapsid from virions
Method: EM (subtomogram averaging) / Resolution: 9.1 Å

EMDB-3874:
Cryo-electron tomogram of Ebola virus
Method: EM (tomography)

EMDB-3875:
The structure of Marburg virus nucleocapsid from virions
Method: EM (subtomogram averaging) / Resolution: 9.1 Å

EMDB-3876:
Cryo-electron tomogram of Marburg virus
Method: EM (tomography)

Source
  • zaire ebolavirus (strain mayinga-76)
  • Ebola virus - Mayinga, Zaire, 1976
  • Marburg virus - Musoke, Kenya, 1980
  • Marburg virus - Angola
KeywordsVIRUS LIKE PARTICLE / nucleocapsid / virus-like particle

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