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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-3875 | ||||||||||||
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Title | The structure of Marburg virus nucleocapsid from virions | ||||||||||||
![]() | Subtomogram averaging of Marburg virus nucleocapsid within intact virions | ||||||||||||
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Biological species | ![]() ![]() ![]() | ||||||||||||
Method | subtomogram averaging / cryo EM / Resolution: 9.1 Å | ||||||||||||
![]() | Wan W / Kolesnikova L / Clarke M / Koehler A / Noda T / Becker S / Briggs JAG | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and assembly of the Ebola virus nucleocapsid. Authors: William Wan / Larissa Kolesnikova / Mairi Clarke / Alexander Koehler / Takeshi Noda / Stephan Becker / John A G Briggs / ![]() ![]() ![]() Abstract: Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles ...Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein-nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment. | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 24.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.4 KB 17.4 KB | Display Display | ![]() |
Images | ![]() | 169.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 264 KB | Display | ![]() |
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Full document | ![]() | 263.2 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3869C ![]() 3870C ![]() 3871C ![]() 3872C ![]() 3873C ![]() 3874C ![]() 3876C ![]() 6ehlC ![]() 6ehmC C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Subtomogram averaging of Marburg virus nucleocapsid within intact virions | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.78 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Lake Victoria marburgvirus - Popp
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Lake Victoria marburgvirus - Popp
Supramolecule | Name: Lake Victoria marburgvirus - Popp / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all Details: Virus was isolated from infected Huh-7 cells. Purified viruses were fixed with paraformaldehyde. NCBI-ID: 33728 / Sci species name: Lake Victoria marburgvirus - Popp / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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Host system | Organism: ![]() |
Virus shell | Shell ID: 1 / Name: Nucleocapsid / Diameter: 310.0 Å |
-Macromolecule #1: Marburg nucleoprotein
Macromolecule | Name: Marburg nucleoprotein / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Sequence | String: MDLHSLLELG TKPTAPHVRN KKVILFDTNH QVSICNQIID AINSGIDLGD LLEGGLLTLC VEHYYNSDK DKFNTSPVAK YLRDAGYEFD VIKNADATRF LDVSPNEPHY SPLILALKTL E STESQRGR IGLFLSFCSL FLPKLVVGDR ASIEKALRQV TVHQEQGIVT ...String: MDLHSLLELG TKPTAPHVRN KKVILFDTNH QVSICNQIID AINSGIDLGD LLEGGLLTLC VEHYYNSDK DKFNTSPVAK YLRDAGYEFD VIKNADATRF LDVSPNEPHY SPLILALKTL E STESQRGR IGLFLSFCSL FLPKLVVGDR ASIEKALRQV TVHQEQGIVT YPNHWLTTGH MK VIFGILR SSFILKFVLI HQGVNLVTGH DAYDSIISNS VGQTRFSGLL IVKTVLEFIL QKT DSGVTL HPLVRTSKVK NEVASFKQAL SNLARHGEYA PFARVLNLSG INNLEHGLYP QLSA IALGV ATAHGSTLAG VNVGEQYQQL REAAHDAEVK LQRRHEHQEI QAIAEDDEER KILEQ FHLQ KTEITHSQTL AVLSQKREKL ARLAAEIENN IVEDQGFKQS QNRVSQSFLN DPTPVE VTV QARPMNRPTA LPPPVDDKIE HESTEDSSSS SSFVDLNDPF ALLNEDEDTL DDSVMIP GT TSREFQGIPE PPRQSQDLNN SQGKQEDEST NRIKKQFLRY QELPPVQEDD ESEYTTDS Q ESIDQPGSDN EQGVDLPPPP LYAQEKRQDP IQHPAANPQD PFGSIGDVNG DILEPIRSP SSPSAPQEDT RMREAYELSP DFTNDEDNQQ NWPQRVVTKK GRTFLYPNDL LQTNPPESLI TALVEEYQN PVSAKELQAD WPDMSFDERR HVAMNL |
-Macromolecule #2: Marburg VP24
Macromolecule | Name: Marburg VP24 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Sequence | String: MAELSTRYNL PANVTENSIN LDLNSTARWI KEPSVGGWTV KWGNFVFHIP NTGMTLLHHL KSNFVVPEW QQTRNLFSHL FKNPKSTIIE PFLALRILLG VALKDQELQQ SLIPGFRSIV H MLSEWLLL EVTSAIHISP NLLGIYLTSD MFKILMAGVK NFFNKMFTLH ...String: MAELSTRYNL PANVTENSIN LDLNSTARWI KEPSVGGWTV KWGNFVFHIP NTGMTLLHHL KSNFVVPEW QQTRNLFSHL FKNPKSTIIE PFLALRILLG VALKDQELQQ SLIPGFRSIV H MLSEWLLL EVTSAIHISP NLLGIYLTSD MFKILMAGVK NFFNKMFTLH VVNDHGKPSS IE IKLTGQQ IIITRVNMGF LVEVRRIDIE PCCGETVLSE SVVFGLVAEA VLREHSQMEK GQP LNLTQY MNSKIAI |
-Macromolecule #3: Marburg VP35
Macromolecule | Name: Marburg VP35 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Sequence | String: MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC LMSKATSTDD IIWDQLIVK RTLADLLIPI NRQISDIQST LSEVTTRVHE IERQLHEITP VLKMGRTLEA I SKGMSEML AKYDHLVIST GRTTAPAAAF DAYLNEHGVP PPQPAIFKDL ...String: MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC LMSKATSTDD IIWDQLIVK RTLADLLIPI NRQISDIQST LSEVTTRVHE IERQLHEITP VLKMGRTLEA I SKGMSEML AKYDHLVIST GRTTAPAAAF DAYLNEHGVP PPQPAIFKDL GVAQQACSKG TM VKNATTD AADKMSKVLE LSEETFSKPN LSAKDLALLL FTHLPGNNTP FHILAQVLSK IAY KSGKSG AFLDAFHQIL SEGENAQAAL TRLSRTFDAF LGVVPPVIRV KNFQTVPRPS QKSL RAVPP NPTIDKGWVC VYSSEQGETR ALKI |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | helical array |
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Sample preparation
Buffer | pH: 7.4 Details: Virus was purified into Dulbecco's modified Eagle's medium (DMEM) with 4% paraformaldehyde |
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Grid | Model: C-flat 2/1 3C / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 20.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Instrument: FEI VITROBOT MARK II |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Lower energy threshold: -10 eV / Energy filter - Upper energy threshold: 10 eV |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 3708 pixel / Digitization - Dimensions - Height: 3708 pixel / Digitization - Frames/image: 1-5 / Average exposure time: 1.0 sec. / Average electron dose: 2.9 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.5 µm / Nominal defocus min: 2.0 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |