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Structure paper

TitleThe structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 10293-10305, Year 2017
Publish dateMay 19, 2017 (structure data deposition date)
AuthorsFranco-Echevarria, E. / Gonzalez-Polo, N. / Zorrilla, S. / Martinez-Lumbreras, S. / Santiveri, C.M. / Campos-Olivas, R. / Sanchez, M. / Calvo, O. / Gonzalez, B. / Perez-Canadillas, J.M.
External linksNucleic Acids Res. / PubMed:28973465
MethodsNMR (solution) / X-ray diffraction
Resolution1.6 - 3.53 Å
Structure data

PDB-5o1t:
Solution structure of the RNA binding domain of Nrd1
Method: SOLUTION NMR

PDB-5o1w:
Structure of Nrd1 RNA binding domain
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5o1x:
Structure of Nrd1 RNA binding domain
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5o1y:
Structure of Nrd1 RNA binding domain in complex with RNA (GUAA)
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-5o1z:
Structure of Nrd1 RNA binding domain in complex with RNA (CGUAAA)
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

PDB-5o20:
Structure of Nrd1 RNA binding domain in complex with RNA (UUAGUAAUCC)
Method: X-RAY DIFFRACTION / Resolution: 3.53 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-SCN:
THIOCYANATE ION

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces (fungus)
KeywordsRNA BINDING PROTEIN / Nrd1 / RRM / RNA-binding / transcription non-coding RNAs / TRANSCRIPTION / Nrd1 complex / non-coding transcription

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