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Structure paper

TitleGuide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 24, Page 825-833, Year 2017
Publish dateJun 3, 2017 (structure data deposition date)
AuthorsKnott, G.J. / East-Seletsky, A. / Cofsky, J.C. / Holton, J.M. / Charles, E. / O'Connell, M.R. / Doudna, J.A.
External linksNat. Struct. Mol. Biol. / PubMed:28892041
MethodsX-ray diffraction
Resolution1.80001033182 - 2.2 Å
Structure data

PDB-5w1h:
Crystal structure of LbaCas13a (C2c2) bound to mature crRNA (24-nt spacer)
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-5w1i:
Crystal structure of LbaCas13a (C2c2) bound to mature crRNA (20-nt spacer)
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5wlh:
Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)
Method: X-RAY DIFFRACTION / Resolution: 1.80001033182 Å

Chemicals

ChemComp-IOD:
IODIDE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • lachnospiraceae bacterium (bacteria)
  • lachnospiraceae bacterium nk4a179 (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / Nuclease RNA binding protein / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex

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