EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
ジャーナル・号・ページ	Nat Methods, Vol. 14, Issue 4, Page 399-402, Year 2017
掲載日	2017年2月13日
著者	M Jason de la Cruz / Johan Hattne / Dan Shi / Paul Seidler / Jose Rodriguez / Francis E Reyes / Michael R Sawaya / Duilio Cascio / Simon C Weiss / Sun Kyung Kim / Cynthia S Hinck / Andrew P Hinck / Guillermo Calero / David Eisenberg / Tamir Gonen /
PubMed 要旨	Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from ...Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
リンク	Nat Methods / PubMed:28192420 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.1 - 2.9 Å
構造データ	8216 5k7n EMDB-8216, PDB-5k7n: MicroED structure of tau VQIVYK peptide at 1.1 A resolution 手法: EM (電子線結晶学) / 解像度: 1.1 Å 8217 5k7o EMDB-8217, PDB-5k7o: MicroED structure of lysozyme at 1.8 A resolution 手法: EM (電子線結晶学) / 解像度: 1.8 Å 8218 5k7p EMDB-8218, PDB-5k7p: MicroED structure of xylanase at 2.3 A resolution 手法: EM (電子線結晶学) / 解像度: 2.3 Å 8219 5k7q EMDB-8219, PDB-5k7q: MicroED structure of thaumatin at 2.5 A resolution 手法: EM (電子線結晶学) / 解像度: 2.5 Å 8220 5k7r EMDB-8220, PDB-5k7r: MicroED structure of trypsin at 1.7 A resolution 手法: EM (電子線結晶学) / 解像度: 1.7 Å 8221 5k7s EMDB-8221, PDB-5k7s: MicroED structure of proteinase K at 1.6 A resolution 手法: EM (電子線結晶学) / 解像度: 1.6 Å 8222 5k7t EMDB-8222, PDB-5k7t: MicroED structure of thermolysin at 2.5 A resolution 手法: EM (電子線結晶学) / 解像度: 2.5 Å 8472 5ty4 EMDB-8472, PDB-5ty4: MicroED structure of a complex between monomeric TGF-b and its receptor, TbRII, at 2.9 A resolution 手法: EM (電子線結晶学) / 解像度: 2.9 Å
化合物	ChemComp-HOH: WATER ChemComp-CL: Unknown entry / クロリド ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-IOD: IODIDE ION / ヨ-ジド ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-DMS: DIMETHYL SULFOXIDE / 2-チアプロパン2-オキシド / DMSO, 沈殿剤YM ChemComp-IPA: ISOPROPYL ALCOHOL / 2-プロパノ-ル / alkaloidYM
由来	homo sapiens (ヒト) gallus gallus (ニワトリ) Chicken (ニワトリ) Trichoderma reesei (菌類) hypocrea jecorina (菌類) thaumatococcus daniellii (植物) Katemfe (植物) bos taurus (ウシ) engyodontium album (菌類) bacillus thermoproteolyticus (バクテリア)
キーワード	PROTEIN FIBRIL / Amyloid / HYDROLASE / PLANT PROTEIN / Sweet protein / TRANSFERASE