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TitleInfluenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.
Journal, issue, pagesMol. Cell, Vol. 61, Page 125-137, Year 2016
Publish dateNov 11, 2015 (structure data deposition date)
AuthorsThierry, E. / Guilligay, D. / Kosinski, J. / Bock, T. / Gaudon, S. / Round, A. / Pflug, A. / Hengrung, N. / El Omari, K. / Baudin, F. ...Thierry, E. / Guilligay, D. / Kosinski, J. / Bock, T. / Gaudon, S. / Round, A. / Pflug, A. / Hengrung, N. / El Omari, K. / Baudin, F. / Hart, D.J. / Beck, M. / Cusack, S.
External linksMol. Cell / PubMed:26711008
MethodsX-ray diffraction
Resolution1.7 - 4.1 Å
Structure data

PDB-5epi:
CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT
Method: X-RAY DIFFRACTION / Resolution: 4.1 Å

PDB-5fml:
Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5fmm:
crystal structure of the mid, cap-binding, mid-link and 627 domains from avian influenza a virus polymerase PB2 subunit bound to M7GTP
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5fmq:
Crystal structure of the mid, cap-binding, mid-link and 627 domains from avian influenza A virus polymerase PB2 subunit bound to M7GTP H32 crystal form
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-5fmz:
Crystal structure of Influenza B polymerase with bound 5' vRNA
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MGT:
7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE

ChemComp-FLC:
CITRATE ANION

Source
  • influenza b virus (b/memphis/13/2003)
  • influenza b virus
  • influenza a virus
KeywordsTRANSFERASE / INFLUENZA B VIRUS / RNA-DEPENDENT RNA POLYMERASE / HETEROTRIMER / SUBUNITS PA / PB1 / PB2 / VIRAL RNA / CRNA 5' END / INFLUENZA / VIRAL PROTEIN / ENDONUCLEASE / INFLUENZA A PB2-C REGION CONTAINING MID / CAP-BINDING / MID-LINK / 627 AND NLS DOMAINS / MID- LINK / TRANSCRIPTION / INFLUENZA B VIRUS RNA-DEPENDENT RNA POLYMERASE / VRNA 5' END.

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