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TitleSampling the conformational space of the catalytic subunit of human γ-secretase.
Journal, issue, pagesElife, Vol. 4, Year 2015
Publish dateDec 1, 2015
AuthorsXiao-chen Bai / Eeson Rajendra / Guanghui Yang / Yigong Shi / Sjors H W Scheres /
PubMed AbstractHuman γ-secretase is an intra-membrane protease that cleaves many different substrates. Aberrant cleavage of Notch is implicated in cancer, while abnormalities in cutting amyloid precursor protein ...Human γ-secretase is an intra-membrane protease that cleaves many different substrates. Aberrant cleavage of Notch is implicated in cancer, while abnormalities in cutting amyloid precursor protein lead to Alzheimer's disease. Our previous cryo-EM structure of γ-secretase revealed considerable disorder in its catalytic subunit presenilin. Here, we describe an image classification procedure that characterizes molecular plasticity at the secondary structure level, and apply this method to identify three distinct conformations in our previous sample. In one of these conformations, an additional transmembrane helix is visible that cannot be attributed to the known components of γ-secretase. In addition, we present a γ-secretase structure in complex with the dipeptidic inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT). Our results reveal how conformational mobility in the second and sixth transmembrane helices of presenilin is greatly reduced upon binding of DAPT or the additional helix, and form the basis for a new model of how substrate enters the transmembrane domain.
External linksElife / PubMed:26623517 / PubMed Central
MethodsEM (single particle)
Resolution4.0 - 4.3 Å
Structure data

EMDB-3237, PDB-5fn2:
Cryo-EM structure of gamma secretase in complex with a drug DAPT
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-3238: Cryo-EM structure of gamma secretase in class 1 of the apo-state ensemble
PDB-5fn3: Cryo-EM structure of gamma secretase in class 1 of the apo- state ensemble
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-3239: Cryo-EM structure of gamma secretase in class 2 of the apo-state ensemble
PDB-5fn4: Cryo-EM structure of gamma secretase in class 2 of the apo- state ensemble
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-3240: Cryo-EM structure of gamma secretase in class 3 of the apo-state ensemble
PDB-5fn5: Cryo-EM structure of gamma secretase in class 3 of the apo- state ensemble
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • homo sapiens (human)
KeywordsHYDROLASE

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