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Structure paper

TitleStructural engineering of a phage lysin that targets Gram-negative pathogens.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 109, Page 9857-9862, Year 2012
Publish dateApr 17, 2012 (structure data deposition date)
AuthorsLukacik, P. / Barnard, T.J. / Keller, P.W. / Chaturvedi, K.S. / Seddiki, N. / Fairman, J.W. / Noinaj, N. / Kirby, T.L. / Henderson, J.P. / Steven, A.C. ...Lukacik, P. / Barnard, T.J. / Keller, P.W. / Chaturvedi, K.S. / Seddiki, N. / Fairman, J.W. / Noinaj, N. / Kirby, T.L. / Henderson, J.P. / Steven, A.C. / Hinnebusch, B.J. / Buchanan, S.K.
External linksProc. Natl. Acad. Sci. USA / PubMed:22679291
MethodsX-ray diffraction
Resolution1.74 - 3.2 Å
Structure data

PDB-4epa:
The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-4epf:
The crystal structure of pesticin from Yersinia pestis
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-4epi:
The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds
Method: X-RAY DIFFRACTION / Resolution: 1.74 Å

PDB-4exm:
The crystal structure of an engineered phage lysin containing the binding domain of pesticin and the killing domain of T4-lysozyme
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-NA:
Unknown entry

Source
  • yersinia pestis (bacteria)
  • enterobacteria phage t4 (virus)
KeywordsMETAL TRANSPORT / tonB dependent transporter / iron import / TOXIN / bacterial toxin / HYDROLASE / bacterial lysin

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