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Title | The random-model method enables ab initio 3D reconstruction of asymmetric particles and determination of particle symmetry. |
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Journal, issue, pages | J Struct Biol, Vol. 171, Issue 2, Page 216-222, Year 2010 |
Publish date | Mar 28, 2010 |
Authors | Eduardo Sanz-García / Aaron B Stewart / David M Belnap / |
PubMed Abstract | Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models ...Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models directly from un-averaged, experimental images of asymmetric or symmetric particles. Therefore, the asymmetric RMM can also be used to determine point-group symmetry. The procedure is facilitated by the use of (a) variable angular step-sizes during iterative origin and orientation searches, (b) high numbers of particle images, and (c) highly defocused images. The method is inhibited by mixed-handedness orientation assignments and by particles with inconspicuous features. For symmetric particles, symmetric RMMs can overcome these deficiencies. |
External links | J Struct Biol / PubMed:20353825 / PubMed Central |
Methods | EM (single particle) |
Resolution | 18.0 - 35.0 Å |
Structure data | EMDB-5141: EMDB-5142: EMDB-5143: EMDB-5144: |
Source |
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