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TitleAtypical chemoreceptor arrays accommodate high membrane curvature.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 5763, Year 2020
Publish dateNov 13, 2020
AuthorsAlise R Muok / Davi R Ortega / Kurni Kurniyati / Wen Yang / Zachary A Maschmann / Adam Sidi Mabrouk / Chunhao Li / Brian R Crane / Ariane Briegel /
PubMed AbstractThe prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended ...The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organization of the histidine kinase CheA in Treponema denticola cells, which possesses arrays with the highest native curvature investigated thus far. Using cryo-ET, we reveal that Td chemoreceptor arrays assume an unusual arrangement of the supra-molecular protein assembly that has likely evolved to accommodate the high membrane curvature. The arrays have several atypical features, such as an extended dimerization domain of CheA and a variant CheW-CheR-like fusion protein that is critical for maintaining an ordered chemosensory apparatus. Furthermore, the previously characterized Td oxygen sensor ODP influences CheA ordering. These results suggest a greater diversity of the chemotaxis signaling system than previously thought.
External linksNat Commun / PubMed:33188180 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography) / X-ray diffraction
Resolution1.5 - 46 Å
Structure data

EMDB-11381:
Treponema denticola chemotaxis signalling arrays in an ODP (TDE2498) deletion mutant
Method: EM (subtomogram averaging) / Resolution: 28.2 Å

EMDB-11384:
Treponema denticola chemotaxis signalling arrays in an ODP (TDE2498) and TDE2496 deletion mutant
Method: EM (subtomogram averaging) / Resolution: 25 Å

EMDB-11385:
Treponema denticola chemotaxis signalling arrays
Method: EM (subtomogram averaging) / Resolution: 16 Å

EMDB-11386:
Treponema denticola chemotaxis signalling arrays
Method: EM (tomography) / Resolution: 46 Å

PDB-6y1y:
CheA dimerization domain of Treponema denticola
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • treponema denticola (bacteria)
KeywordsAmino Acid Sequence / Bacterial Proteins / Cell Membrane / Chemoreceptor Cells / Chemotaxis / Conserved Sequence / Escherichia coli / Gene Deletion / Histidine Kinase / Protein Domains / Sequence Homology, Amino Acid / Treponema / SIGNALING PROTEIN / dimerization domain / coiled-coil

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