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TitleStructural basis of transcription-translation coupling and collision in bacteria.
Journal, issue, pagesScience, Vol. 369, Issue 6509, Page 1355-1359, Year 2020
Publish dateSep 11, 2020
AuthorsMichael William Webster / Maria Takacs / Chengjin Zhu / Vita Vidmar / Ayesha Eduljee / Mo'men Abdelkareem / Albert Weixlbaumer /
PubMed AbstractProkaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The ...Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures representing uncoupled, coupled, and collided expressome states determined by cryo-electron microscopy. A bridge between the ribosome and RNAP can be formed by the transcription factor NusG, which stabilizes an otherwise-variable interaction interface. Shortening of the intervening mRNA causes a substantial rearrangement that aligns the ribosome entrance channel to the RNAP exit channel. In this collided complex, NusG linkage is no longer possible. These structures reveal mechanisms of coordination between transcription and translation and provide a framework for future study.
External linksScience / PubMed:32820062
MethodsEM (single particle)
Resolution3 - 3.9 Å
Structure data

EMDB-11418, PDB-6ztj:
E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-11419, PDB-6ztl:
E. coli 70S-RNAP expressome complex in collided state bound to NusG
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-11420, PDB-6ztm:
E. coli 70S-RNAP expressome complex in collided state without NusG
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-11421, PDB-6ztn:
E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-11422, PDB-6zto:
E. coli 70S-RNAP expressome complex in uncoupled state 1
Method: EM (single particle) / Resolution: 3 Å

EMDB-11423, PDB-6ztp:
E. coli 70S-RNAP expressome complex in uncoupled state 6
Method: EM (single particle) / Resolution: 3 Å

EMDB-11426, PDB-6zu1:
E. coli 70S-RNAP expressome complex in uncoupled state 2
Method: EM (single particle) / Resolution: 3 Å

Chemicals

ChemComp-MG:
MAGNESIUM ION / Magnesium

ChemComp-PHE:
PHENYLALANINE / Phenylalanine

ChemComp-ZN:
ZINC ION / Zinc

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsGENE REGULATION / Transcription / Translation / Expressome / Ribosome / RNA polymerase

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