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Structure paper

TitleStructure of nucleosome-bound human BAF complex.
Journal, issue, pagesScience, Year 2020
Publish dateJan 30, 2020
AuthorsShuang He / Zihan Wu / Yuan Tian / Zishuo Yu / Jiali Yu / Xinxin Wang / Jie Li / Bijun Liu / Yanhui Xu /
PubMed AbstractMammalian SWI/SNF family chromatin remodelers, BAF and PBAF, regulate chromatin structure and transcription, with their mutations linked to cancers. The 3.7 Å-resolution cryo-EM structure of human ...Mammalian SWI/SNF family chromatin remodelers, BAF and PBAF, regulate chromatin structure and transcription, with their mutations linked to cancers. The 3.7 Å-resolution cryo-EM structure of human BAF bound to nucleosome reveals that the nucleosome is sandwiched by the Base and the ATPase modules, which are bridged by the actin-related protein (ARP) module. The ATPase motor is positioned proximal to nucleosomal DNA and, upon ATP hydrolysis, would engage with and pump DNA along the nucleosome. The C-terminal α-helix of SMARCB1, enriched in positively charged residues frequently mutated in cancers, mediates interactions with an acidic patch of nucleosome. ARID1A and SMARCC serve as a structural core and scaffold in the Base module organization, respectively. Our study provides structural insights into subunit organization and nucleosome recognition of human BAF complex.
External linksPubMed:32001526 / Publisher's page
KeywordsGENE REGULATION / Chromatin remodeler / Complex
MethodsEM (single particle)
Resolution3 - 10.3 A
Structure data

EMDB-0968:
Structure of human BAF Base module

EMDB-0969:
Structure of human BAF ARP module

EMDB-0970:
Structure of nucleosome in BAF-NCP complex

EMDB-0971:
Structure of nucleosome-bound human full length BAF complex

EMDB-0972:
Structure of nucleosome with DNA detachment in BAF-NCP complex

EMDB-0973:
Structure of nucleosome-bound human BAF complex in ADP-bound state

EMDB-0974:
Structure of nucleosome-bound human BAF complex

PDB-6lth:
Structure of human BAF Base module

PDB-6ltj:
Structure of nucleosome-bound human BAF complex

Chemicals

ChemComp-ZN:
ZINC ION / Zinc

Source
  • homo sapiens (human)
  • Xenopus calcaratus (Biafran clawed frog)
  • xenopus laevis (African clawed frog)

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