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Structure paper

タイトル	Influence of lipid bilayer on the structure of the muscle-type nicotinic acetylcholine receptor.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 121, Issue 19, Page e2319913121, Year 2024
掲載日	2024年5月7日
著者	Nigel Unwin /
PubMed 要旨	The muscle-type nicotinic acetylcholine receptor is a transmitter-gated ion channel residing in the plasma membrane of electrocytes and striated muscle cells. It is present predominantly at synaptic ...The muscle-type nicotinic acetylcholine receptor is a transmitter-gated ion channel residing in the plasma membrane of electrocytes and striated muscle cells. It is present predominantly at synaptic junctions, where it effects rapid depolarization of the postsynaptic membrane in response to acetylcholine released into the synaptic cleft. Previously, cryo-EM of intact membrane from revealed that the lipid bilayer surrounding the junctional receptor has a uniquely asymmetric and ordered structure, due to a high concentration of cholesterol. It is now shown that this special lipid environment influences the transmembrane (TM) folding of the protein. All five submembrane MX helices of the membrane-intact junctional receptor align parallel to the surface of the cholesterol-ordered lipids in the inner leaflet of the bilayer; also, the TM helices in the outer leaflet are splayed apart. However in the structure obtained from the same protein after extraction and incorporation in nanodiscs, the MX helices do not align to a planar surface, and the TM helices arrange compactly in the outer leaflet. Realignment of the MX helices of the nanodisc-solved structure to a planar surface converts their adjoining TM helices into an obligatory splayed configuration, characteristic of the junctional receptor. Thus, the form of the receptor sustained by the special lipid environment of the synaptic junction is the one that mediates fast synaptic transmission; whereas, the nanodisc-embedded protein may be like the extrajunctional form, existing in a disordered lipid environment.
リンク	Proc Natl Acad Sci U S A / PubMed:38683987 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	4.7 - 9.4 Å
構造データ	18596 8qqm EMDB-18596, PDB-8qqm: nicotinic acetylcholine receptor in intact synaptic membrane 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-18785: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-18802: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.6 Å EMDB-18804: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.0 Å EMDB-18805: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.0 Å EMDB-18816: Influence of lipid bilayer on structure of acetylcholine recptor 手法: EM (らせん対称) / 解像度: 8.8 Å EMDB-18817: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.8 Å EMDB-18823: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.3 Å EMDB-18824: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.7 Å EMDB-18831: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-18832: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.6 Å EMDB-18835: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.0 Å EMDB-18836: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.9 Å EMDB-18837: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.6 Å EMDB-18838: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.7 Å EMDB-18840: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.3 Å EMDB-18843: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.9 Å EMDB-18844: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.4 Å EMDB-18845: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.8 Å EMDB-18846: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.9 Å EMDB-18847: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-18849: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.2 Å EMDB-18850: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.7 Å EMDB-18853: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.9 Å EMDB-18854: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.1 Å EMDB-18855: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.4 Å EMDB-18856: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.7 Å EMDB-18857: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-18858: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.6 Å EMDB-18862: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.8 Å EMDB-18863: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.1 Å EMDB-18865: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-18867: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 8.2 Å EMDB-18869: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.0 Å EMDB-18870: Influence of lipid bilayer on structure of acetylcholine receptor 手法: EM (らせん対称) / 解像度: 9.1 Å
由来	Torpedo marmorata (エイ) tetronarce californica (ゴマフシビレエイ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Ion channel (イオンチャネル)