Movie
Controller
Structure viewers
About EMN Papers
+Search query
-Structure paper
| Title | Fine structure and assembly pattern of a minimal myophage Pam3. |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 120, Issue 4, Page e2213727120, Year 2023 |
| Publish date | Jan 24, 2023 |
 Authors | Feng Yang / Yong-Liang Jiang / Jun-Tao Zhang / Jie Zhu / Kang Du / Rong-Cheng Yu / Zi-Lu Wei / Wen-Wen Kong / Ning Cui / Wei-Fang Li / Yuxing Chen / Qiong Li / Cong-Zhao Zhou /  |
| PubMed Abstract | The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail ...The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. |
 External links | Proc Natl Acad Sci U S A / PubMed:36656854 / PubMed Central |
| Methods | EM (single particle) / EM (helical sym.) |
| Resolution | 3.0 - 3.96 Å |
| Structure data | EMDB-33799, PDB-7yfw: EMDB-33802, PDB-7yfz: EMDB-34678, PDB-8hdr: EMDB-34679, PDB-8hds: EMDB-34680, PDB-8hdt: EMDB-34681, PDB-8hdw: |
| Source |
|
 Keywords |  VIRAL PROTEIN / fiber / VIRUS / baseplate / portal-adaptor / capsid asymmetric unit / Sheath-tube |
uncultured cyanophage (environmental samples)
