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| Title | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant. |
|---|---|
| Journal, issue, pages | Nat Commun, Vol. 9, Issue 1, Page 3628, Year 2018 |
| Publish date | Sep 7, 2018 |
 Authors | Yong Zi Tan / Sriram Aiyer / Mario Mietzsch / Joshua A Hull / Robert McKenna / Joshua Grieger / R Jude Samulski / Timothy S Baker / Mavis Agbandje-McKenna / Dmitry Lyumkis /  |
| PubMed Abstract | Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures ...Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more standard as resolutions increase or at lower microscope accelerating voltages. The combined application of the described methods to micrographs recorded on a Titan Krios enables structure determination at ~1.86-Å resolution of an adeno-associated virus serotype 2 variant (AAV2), an important gene-delivery vehicle. The resulting structural details provide an improved model for understanding the biology of AAV that will guide future vector development for gene therapy. |
 External links | Nat Commun / PubMed:30194371 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 1.86 Å |
| Structure data | |
| Chemicals |  ChemComp-HOH: |
| Source |
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 Keywords |  VIRUS LIKE PARTICLE / AAV-2 L336C / Gene therapy / Ewald sphere curvature correction / Per particle CTF |
