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Structure paper

タイトル	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.
ジャーナル・号・ページ	Nat Commun, Vol. 9, Issue 1, Page 3628, Year 2018
掲載日	2018年9月7日
著者	Yong Zi Tan / Sriram Aiyer / Mario Mietzsch / Joshua A Hull / Robert McKenna / Joshua Grieger / R Jude Samulski / Timothy S Baker / Mavis Agbandje-McKenna / Dmitry Lyumkis /
PubMed 要旨	Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures ...Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more standard as resolutions increase or at lower microscope accelerating voltages. The combined application of the described methods to micrographs recorded on a Titan Krios enables structure determination at ~1.86-Å resolution of an adeno-associated virus serotype 2 variant (AAV2), an important gene-delivery vehicle. The resulting structural details provide an improved model for understanding the biology of AAV that will guide future vector development for gene therapy.
リンク	Nat Commun / PubMed:30194371 / PubMed Central
手法	EM (単粒子)
解像度	1.86 Å
構造データ	9012 6e9d EMDB-9012: Sub-2 Angstrom Ewald Curvature Corrected Single-Particle Cryo-EM Reconstruction of AAV-2 L336C PDB-6e9d: Sub-2 Angstrom Ewald Curvature-Corrected Single-Particle Cryo-EM Reconstruction of AAV-2 L336C 手法: EM (単粒子) / 解像度: 1.86 Å
化合物	ChemComp-HOH: WATER / 水
由来	adeno-associated virus 2 srivastava/1982 (アデノ随伴ウイルス)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / AAV-2 L336C / Gene therapy (遺伝子治療) / Ewald sphere curvature correction / Per particle CTF