Katharina Braunger / Stefan Pfeffer / Shiteshu Shrimal / Reid Gilmore / Otto Berninghausen / Elisabet C Mandon / Thomas Becker / Friedrich Förster / Roland Beckmann /
PubMed Abstract
Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and ...Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and oligosaccharyltransferase (OST). Here we used different cryo-electron microscopy approaches to determine structures of native and solubilized ribosome-Sec61-OST complexes. A molecular model for the catalytic OST subunit STT3A (staurosporine and temperature sensitive 3A) revealed how it is integrated into the OST and how STT3-paralog specificity for translocon-associated OST is achieved. The OST subunit DC2 was placed at the interface between Sec61 and STT3A, where it acts as a versatile module for recruitment of STT3A-containing OST to the ribosome-Sec61 complex. This detailed structural view on the molecular architecture of the cotranslational machinery for N-glycosylation provides the basis for a mechanistic understanding of glycoprotein biogenesis at the endoplasmic reticulum.
EMDB-4306: Subtomogram average of unsorted ribosome-translocon complexes from STT3B(-/-) HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4307: Subtomogram average of OST-free ribosome-translocon complexes from STT3B(-/-) HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4308: Subtomogram average of OST-containing ribosome-translocon complexes from STT3B(-/-) HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4309: Subtomogram average of unsorted ribosome-translocon complexes from STT3A(-/-) HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4310: Subtomogram average of OST-free ribosome-translocon complexes from STT3A(-/-) HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4311: Subtomogram average of ribosome-translocon complexes from STT3A(-/-) HEK cells containing an unidentified ER-lumenal component Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4312: Subtomogram average of unsorted ribosome-translocon complexes from wild type HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4313: Subtomogram average of OST-free ribosome-translocon complexes from wild type HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4314: Subtomogram average of OST-containing ribosome-translocon complexes from wild type HEK cells Method: EM (subtomogram averaging) / Resolution: 30.0 Å
EMDB-4315, PDB-6ftg: Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes Method: EM (subtomogram averaging) / Resolution: 9.1 Å
EMDB-4316, PDB-6fti: Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome Method: EM (single particle) / Resolution: 4.2 Å
EMDB-4317, PDB-6ftj: Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome Method: EM (single particle) / Resolution: 4.7 Å
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels
Nobel Prize for mechanically activated and temperature-gated ion channels
The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
EM Navigator can help to find cryo-EM structure data by both pioneers.
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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PROTEIN TRANSPORT / Protein translocon of the endoplasmic reticulum
