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Structure paper

TitleA structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 11, Page 931-943, Year 2017
Publish dateOct 9, 2017
AuthorsJoseph Atherton / Kai Jiang / Marcel M Stangier / Yanzhang Luo / Shasha Hua / Klaartje Houben / Jolien J E van Hooff / Agnel-Praveen Joseph / Guido Scarabelli / Barry J Grant / Anthony J Roberts / Maya Topf / Michel O Steinmetz / Marc Baldus / Carolyn A Moores / Anna Akhmanova /
PubMed AbstractCAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization ...CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we found that the CAMSAP C-terminal CKK domain is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncovered how mammalian CKK binds between two tubulin dimers at the interprotofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high-resolution fluorescence microscopy and cryo-electron tomography suggested that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the interprotofilament interface at this site serve as the basis for CKK's minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends.
External linksNat Struct Mol Biol / PubMed:28991265 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.4 - 8.0 Å
Structure data

3444

5m5c

EMDB-3444, PDB-5m5c:
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Method: EM (single particle) / Resolution: 4.8 Å

4154

5m50

EMDB-4154, PDB-5m50:
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Method: EM (single particle) / Resolution: 5.3 Å

4156

5m54

EMDB-4156, PDB-5m54:
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Method: EM (single particle) / Resolution: 8.0 Å

PDB-5lzn:
-TIP microtubule-binding domain
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • bos taurus (cattle)
  • Homo sapiens (human)
  • Bovine (cattle)
  • mus musculus (house mouse)
KeywordsSTRUCTURAL PROTEIN / -TIP / microtubule / CAMSAP CKK Microtubule Tubulin / MOTOR PROTEIN / CAMSAP / CKK / Tubulin / TRANSPORT PROTEIN

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